Conjugated linoleic acid isomers inhibit platelet-derived growth factor-induced NF-κB transactivation and collagen formation in human vascular smooth muscle cells - Research portal of Justus Liebig University Giessen:

Journal article

Conjugated linoleic acid isomers inhibit platelet-derived growth factor-induced NF-κB transactivation and collagen formation in human vascular smooth muscle cells

Authors list: Ringseis, R; Gahler, S; Eder, K

Publication year: 2008

Pages: 59-67

Journal: European Journal of Nutrition

Volume number: 47

Issue number: 2

ISSN: 1436-6207

DOI Link: https://doi.org/10.1007/s00394-008-0697-8

Publisher: Springer

Abstract:
Background: Atherosclerosis is characterized by extensive thickening of the arterial intima partially resulting from deposition of collagen by vascular smooth muscle cells (SMCs). Polyunsaturated fatty acids stimulate collagen formation through NF-kappa B activation.
Aim of the study: The present study aimed to explore the effect of conjugated linoleic acids (CLAs) which are known to inhibit NF-kappa B activation on collagen formation by SMCs.
Methods: Vascular SMCs were cultured with 50 mu mol/l of CLA isomers (c9t11-CLA, t10c12-CLA) or linoleic acid (LA) and analysed for collagen formation and NF-kappa B p50 transactivation.
Results: Treatment with CLA isomers but not LA significantly reduced PDGF-stimulated [H-3] proline incorporation into cell layer protein of SMCs without altering cell proliferation. Simultaneous treatment with the PPAR gamma inhibitor T0070907 abrogated this effect. Treatment of SMCs with c9t11-CLA and t10c12-CLA significantly reduced PDGF-induced NF-kappa B p50 activation.
Conclusions: CLA isomers inhibit PDGF-stimulated collagen production by vascular SMCs, which is considered to be a hallmark of atherosclerosis, in a PPAR gamma-dependent manner. Whether inhibition of the NF-kappa B-pathway is of significance for the reduction of collagen formation by CLA isomers needs further investigation.

Authors/Editors

- Uni.-Prof. Dr. Klaus Josef Eder

Citation Styles

Harvard Citation style: Ringseis, R., Gahler, S. and Eder, K. (2008) Conjugated linoleic acid isomers inhibit platelet-derived growth factor-induced NF-κB transactivation and collagen formation in human vascular smooth muscle cells, European Journal of Nutrition, 47(2), pp. 59-67. https://doi.org/10.1007/s00394-008-0697-8

APA Citation style: Ringseis, R., Gahler, S., & Eder, K. (2008). Conjugated linoleic acid isomers inhibit platelet-derived growth factor-induced NF-κB transactivation and collagen formation in human vascular smooth muscle cells. European Journal of Nutrition. 47(2), 59-67. https://doi.org/10.1007/s00394-008-0697-8