Journal article

Publication year: 2014

Pages: 345-349

Journal: Journal of Dairy Science

Volume number: 97

Issue number: 1

ISSN: 0022-0302

Open access status: Bronze

DOI Link: https://doi.org/10.3168/jds.2013-7161

Publisher: Elsevier

Abstract: 
Recent studies in rodents demonstrated that peroxisome proliferator-activated receptor alpha (PPAR alpha), a central regulator of energy homeostasis, is an important transcriptional regulator of the gene encoding the carnitine transporter novel organic cation transporter 2 (OCTN2). Less is known with regard to the regulation of OCTN2 by PPAR alpha and its role for carnitine transport in cattle, even though PPAR alpha activation physiologically occurs in the liver of high-producing cows during early lactation. To explore the role of PPAR alpha. for OCTN2 expression and carnitine transport in cattle, we studied the effect of the PPAR alpha activator WY-14,643 on the expression of OCTN2 in the presence and absence of PPAR alpha antagonists and on OCTN2-mediated carnitine transport in the Madin-Darby bovine kidney (MDBK) cell line. The results show that WY-14,643 increases mRNA and protein levels of OCTN2, whereas co-treatment of MDBK cells with WY-14,643 and the PPAR alpha antagonist GW6471 blocks the WY-14,643-induced increase in mRNA and protein levels of OCTN2 in bovine cells. In addition, treatment of MDBK cells with WY-14,643 stimulates specifically Na+-dependent carnitine uptake in MDBK cells, which is likely the consequence of the increased carnitine transport capacity of cells due to the elevated expression of OCTN2. In conclusion, our results indicate that OCTN2 expression and carnitine transport in cattle, as in rodents, are regulated by PPAR alpha.

Harvard Citation style: Zhou, X., Wen, G., Ringseis, R. and Eder, K. (2014) The pharmacological peroxisome proliferator-activated receptor alpha agonist WY-14,643 increases expression of novel organic cation transporter 2 and carnitine uptake in bovine kidney cells, Journal of Dairy Science, 97(1), pp. 345-349. https://doi.org/10.3168/jds.2013-7161

APA Citation style: Zhou, X., Wen, G., Ringseis, R., & Eder, K. (2014). The pharmacological peroxisome proliferator-activated receptor alpha agonist WY-14,643 increases expression of novel organic cation transporter 2 and carnitine uptake in bovine kidney cells. Journal of Dairy Science. 97(1), 345-349. https://doi.org/10.3168/jds.2013-7161