Journal article
Authors list: Missirlis, F; Ulschmid, JK; Hirosawa-Takamori, M; Grönke, S; Schäfer, U; Becker, K; Phillips, JP; Jäckle, H
Publication year: 2002
Pages: 11521-11526
Journal: Journal of Biological Chemistry
Volume number: 277
Issue number: 13
ISSN: 0021-9258
eISSN: 1083-351X
Open access status: Hybrid
DOI Link: https://doi.org/10.1074/jbc.M111692200
Publisher: Elsevier
Abstract:
Defense against oxidative stress in mammals includes the regeneration of the major thiol reductants glutathione and thioredoxin by glutathione reductase and thioredoxin reductase (TrxR), respectively. In contrast, Drosophila, and possibly insects in general, lacks glutathione reductase and must rely solely on the TrxR system. The mammalian TrxRs described so far are selenoproteins that utilize NADPH to reduce protein as well as nonprotein substrates in mitochondria and cytoplasm of cells. We show that a single Drosophila gene, Trxr-1, encodes non-selenocysteine-containing cytoplasmic and mitochondrial TrxR isoforms that differ with respect to their N termini. We generated transcript-specific mutants and used in vivo approaches to explore the biological functions of the two enzyme variants by introducing the corresponding transgenes into different Trxr-1 mutants. The results show that, although the two TrxR isoforms have similar biochemical properties, their biological functions are not interchangeable.
Citation Styles
Harvard Citation style: Missirlis, F., Ulschmid, J., Hirosawa-Takamori, M., Grönke, S., Schäfer, U., Becker, K., et al. (2002) Mitochondrial and cytoplasmic thioredoxin reductase variants encoded by a single Drosophila gene are both essential for viability, Journal of Biological Chemistry, 277(13), pp. 11521-11526. https://doi.org/10.1074/jbc.M111692200
APA Citation style: Missirlis, F., Ulschmid, J., Hirosawa-Takamori, M., Grönke, S., Schäfer, U., Becker, K., Phillips, J., & Jäckle, H. (2002). Mitochondrial and cytoplasmic thioredoxin reductase variants encoded by a single Drosophila gene are both essential for viability. Journal of Biological Chemistry. 277(13), 11521-11526. https://doi.org/10.1074/jbc.M111692200