Journal article

Mitochondrial and cytoplasmic thioredoxin reductase variants encoded by a single Drosophila gene are both essential for viability


Authors listMissirlis, F; Ulschmid, JK; Hirosawa-Takamori, M; Grönke, S; Schäfer, U; Becker, K; Phillips, JP; Jäckle, H

Publication year2002

Pages11521-11526

JournalJournal of Biological Chemistry

Volume number277

Issue number13

ISSN0021-9258

eISSN1083-351X

Open access statusHybrid

DOI Linkhttps://doi.org/10.1074/jbc.M111692200

PublisherElsevier


Abstract
Defense against oxidative stress in mammals includes the regeneration of the major thiol reductants glutathione and thioredoxin by glutathione reductase and thioredoxin reductase (TrxR), respectively. In contrast, Drosophila, and possibly insects in general, lacks glutathione reductase and must rely solely on the TrxR system. The mammalian TrxRs described so far are selenoproteins that utilize NADPH to reduce protein as well as nonprotein substrates in mitochondria and cytoplasm of cells. We show that a single Drosophila gene, Trxr-1, encodes non-selenocysteine-containing cytoplasmic and mitochondrial TrxR isoforms that differ with respect to their N termini. We generated transcript-specific mutants and used in vivo approaches to explore the biological functions of the two enzyme variants by introducing the corresponding transgenes into different Trxr-1 mutants. The results show that, although the two TrxR isoforms have similar biochemical properties, their biological functions are not interchangeable.



Citation Styles

Harvard Citation styleMissirlis, F., Ulschmid, J., Hirosawa-Takamori, M., Grönke, S., Schäfer, U., Becker, K., et al. (2002) Mitochondrial and cytoplasmic thioredoxin reductase variants encoded by a single Drosophila gene are both essential for viability, Journal of Biological Chemistry, 277(13), pp. 11521-11526. https://doi.org/10.1074/jbc.M111692200

APA Citation styleMissirlis, F., Ulschmid, J., Hirosawa-Takamori, M., Grönke, S., Schäfer, U., Becker, K., Phillips, J., & Jäckle, H. (2002). Mitochondrial and cytoplasmic thioredoxin reductase variants encoded by a single Drosophila gene are both essential for viability. Journal of Biological Chemistry. 277(13), 11521-11526. https://doi.org/10.1074/jbc.M111692200


Last updated on 2025-10-06 at 09:26