PPARγ Ligand Troglitazone Lowers Cholesterol Synthesis in HepG2 and Caco-2 Cells via a Reduced Concentration of Nuclear SREBP-2 - Research portal of Justus Liebig University Giessen:

Journal article

PPARγ Ligand Troglitazone Lowers Cholesterol Synthesis in HepG2 and Caco-2 Cells via a Reduced Concentration of Nuclear SREBP-2

Authors list: Klopotek, A; Hirche, F; Eder, K

Publication year: 2006

Pages: 1365-1372

Journal: Experimental Biology and Medicine

Volume number: 231

Issue number: 8

ISSN: 1535-3702

DOI Link: https://doi.org/10.1177/153537020623100810

Publisher: Frontiers Media

Abstract:
Cholesterol synthesis in animal cells is regulated by sterol regulatory element-binding protein (SREBP)-2. The objective of this study was to investigate whether activation of peroxisome proliferator-activated receptor (PPAR)-gamma influences the SREBP-2 dependent cholesterol synthesis in liver and intestinal cells. Therefore, HepG2 and Caco-2 cells were incubated with and without 10 or 30 mu M of troglitazone, a synthetic PPAR gamma agonist, for 4 hrs. Incubation with 10 or 30 mu M of troglitazone caused a significant, dose-dependent reduction of cholesterol synthesis in both HepG2 and Caco-2 cells (P < 0.05). HepG2 and Caco-2 cells incubated with 10 or 30 mu M of troglitazone had also lower mRNA concentrations and lower nuclear protein concentrations of SREBP-2 than untreated control cells (P < 0.05). mRNA concentrations of the SREBP-2 target genes HMG-CoA reductase and LDL receptor were also reduced in HepG2 and Caco-2 cells treated with 30 mu M of troglitazone compared to control cells (P < 0.05). In conclusion, this study shows that PPAR gamma activation by troglitazone lowers the cholesterol synthesis in HepG2 and Caco-2 cells by reducing the concentration of nuclear SREBP-2 and successive downregulation of its target genes involved in cholesterol synthesis.

Authors/Editors

- Uni.-Prof. Dr. Klaus Josef Eder

Citation Styles

Harvard Citation style: Klopotek, A., Hirche, F. and Eder, K. (2006) PPARγ Ligand Troglitazone Lowers Cholesterol Synthesis in HepG2 and Caco-2 Cells via a Reduced Concentration of Nuclear SREBP-2, Experimental Biology and Medicine, 231(8), pp. 1365-1372. https://doi.org/10.1177/153537020623100810

APA Citation style: Klopotek, A., Hirche, F., & Eder, K. (2006). PPARγ Ligand Troglitazone Lowers Cholesterol Synthesis in HepG2 and Caco-2 Cells via a Reduced Concentration of Nuclear SREBP-2. Experimental Biology and Medicine. 231(8), 1365-1372. https://doi.org/10.1177/153537020623100810