Coincident enrichment of phosphorylated I kappa B alpha, activated IKK, and phosphorylated p65 in the axon initial segment of neurons - Research portal of Justus Liebig University Giessen:

Journal article

Coincident enrichment of phosphorylated I kappa B alpha, activated IKK, and phosphorylated p65 in the axon initial segment of neurons

Authors list: Schultz, C; König, HG; Del Turco, D; Politi, C; Eckert, GP; Ghebremedhin, E; Prehn, JHM; Kögel, D; Deller, T

Publication year: 2006

Pages: 68-80

Journal: Molecular and Cellular Neuroscience

Volume number: 33

Issue number: 1

ISSN: 1044-7431

DOI Link: https://doi.org/10.1016/j.mcn.2006.06.008

Publisher: Elsevier

Abstract:
Phosphorylation of the inhibitory protein I kappa B alpha by the activated I kappa B kinase (IKK) is a crucial step in the activation of the transcription factor NF-kappa B. In neurons of the mammalian central nervous system, constitutive activation of NF-kappa B has been previously documented. The cellular compartments involved in this activation have not yet been fully identified. Here we document a striking enrichment of several molecules involved in NF-kappa B activation in the axon initial segment (AIS) of neurons: Phosphorylated-I kappa B alpha (pI kappa B alpha), activated IKK, and p65 phosphorylated at serine 536 were found to be enriched in the AIS in vivo as well as in vitro. Both, pI kappa B alpha, and activated IKK, were associated with cytoskeletal components of the AIS. Activated IKK was associated with the membrane cytoskeleton, whereas pI kappa B alpha was sequestered to microtubules of the AIS. Colchicine-induced depolymerization of microtubules resulted in the loss of pI kappa B alpha in the AIS, demonstrating that the integrity of the axonal cytoskeleton is essential for the clustering of this NF-kappa B pathway component. These data provide the first evidence for a compartmentalized clustering of NF-kappa B pathway components in the AIS and implicate this neuronal compartment in the activation of NF-kappa B.

Citation Styles

Harvard Citation style: Schultz, C., König, H., Del Turco, D., Politi, C., Eckert, G., Ghebremedhin, E., et al. (2006) Coincident enrichment of phosphorylated I kappa B alpha, activated IKK, and phosphorylated p65 in the axon initial segment of neurons, Molecular and Cellular Neuroscience, 33(1), pp. 68-80. https://doi.org/10.1016/j.mcn.2006.06.008

APA Citation style: Schultz, C., König, H., Del Turco, D., Politi, C., Eckert, G., Ghebremedhin, E., Prehn, J., Kögel, D., & Deller, T. (2006). Coincident enrichment of phosphorylated I kappa B alpha, activated IKK, and phosphorylated p65 in the axon initial segment of neurons. Molecular and Cellular Neuroscience. 33(1), 68-80. https://doi.org/10.1016/j.mcn.2006.06.008