L-cysteine down-regulates SREBP-1c-regulated lipogenic enzymes expression via glutathione in HepG2 cells - Research portal of Justus Liebig University Giessen:

Journal article

L-cysteine down-regulates SREBP-1c-regulated lipogenic enzymes expression via glutathione in HepG2 cells

Authors list: Bettzieche, A; Brandsch, C; Hirche, F; Eder, K; Stangl, GI

Publication year: 2008

Pages: 196-203

Journal: Annals of Nutrition and Metabolism

Volume number: 52

Issue number: 3

ISSN: 0250-6807

DOI Link: https://doi.org/10.1159/000138123

Publisher: Karger Publishers

Abstract:
Background/Aim: Protein-associated amino acids are supposed to play a role in sterol regulatory element-binding protein (SREBP)-mediated regulation of lipid metabolism. This study investigates the effects of cysteine on expression of SREBP-regulated hepatic genes.
Methods: HepG2 cells which are an accepted model for the study of the lipid metabolism were treated with L-cysteine under different conditions.
Results: Exposure of cells to L-cysteine reduced the mRNA concentrations of SREBP-1c (-35 to-43%) and its target genes fatty acid synthase (FAS;-20 to-50%), glucose-6-phosphate- dehydrogenase (G6PDH;-31 to-35%), and stearoyl-coenzyme A desaturase (SCD) 1 (-34 to-50%). Cells treated with L-cysteine had 47% higher glutathione and 47% lower triglyceride concentrations than control cells. In cells which were concurrently treated with L-cysteine and L-buthionine[ S, R]-sulfoximine, an inhibitor of enzymatic glutathione synthesis, no down-regulation of the gene expression was observed. Pro-oxidant CuSO4 up-regulated SREBP-1c (+ 71%), FAS (+ 165%), G6PDH (+ 84%) and SCD1 (+ 96%) mRNA abundance compared to control cells, but when cells were concurrently treated with L-cysteine, the gene expression remained at control level.
Conclusions: The results show that L-cysteine rapidly down-regulates the transcription of genes involved in fatty acid biosynthesis via a mechanism that appears to be mediated by an improved glutathione status.

Authors/Editors

- Uni.-Prof. Dr. Klaus Josef Eder

Citation Styles

Harvard Citation style: Bettzieche, A., Brandsch, C., Hirche, F., Eder, K. and Stangl, G. (2008) L-cysteine down-regulates SREBP-1c-regulated lipogenic enzymes expression via glutathione in HepG2 cells, Annals of Nutrition and Metabolism, 52(3), pp. 196-203. https://doi.org/10.1159/000138123

APA Citation style: Bettzieche, A., Brandsch, C., Hirche, F., Eder, K., & Stangl, G. (2008). L-cysteine down-regulates SREBP-1c-regulated lipogenic enzymes expression via glutathione in HepG2 cells. Annals of Nutrition and Metabolism. 52(3), 196-203. https://doi.org/10.1159/000138123