Journalartikel

Jahr der Veröffentlichung: 2008

Seiten: 946-952

Zeitschrift: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Bandnummer: 1784

Heftnummer: 6

ISSN: 1570-9639

DOI Link: https://doi.org/10.1016/j.bbapap.2008.03.012

Verlag: Elsevier

Abstract: 
Recently discovered monothiol glutaredoxins with CXXS-active site sequence share a common structural motif and biochemical mechanism of action and are involved in multiple cellular functions. Here we report first studies on the structural and stability characterization of a monothiol glutaredoxin, in particular - PfGLP1. Our results demonstrate that in the native conformation, the enzyme has a compact core structure with a relatively flexible N-terminal portion having an open configuration. Comparative functional studies with the full-length and N-terminal truncated protein demonstrate that the flexible N-terminal portion does not play any significant role in functional activity of the protein. In contrast to other Grxs, PfGLP1 does not contain a Fe-S cluster. The pH dependent studies demonstrate that the protein is resistant to alkaline pH but highly sensitive to acidic pH and undergoes significant unfolding between pH 4 and 5. However, acidic conditions also do not induce complete unfolding of the enzyme. The protein is stabilized with a conformational free energy of about 3.2 +/- 0.1 kcal mol(-1). The protein is a highly cooperative molecule as during denaturant-induced equilibrium unfolding a simultaneous unfolding of the protein without stabilization of any partially folded intermediate is observed. (C) 2008 Elsevier B.V. All rights reserved.

Harvard-Zitierstil: Tripathi, T., Rahlfs, S., Becker, K. and Bhakuni, V. (2008) Structural and stability characteristics of a monothiol glutaredoxin: Glutaredoxin-like protein 1 from Plasmodium falciparum, Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1784(6), pp. 946-952. https://doi.org/10.1016/j.bbapap.2008.03.012

APA-Zitierstil: Tripathi, T., Rahlfs, S., Becker, K., & Bhakuni, V. (2008). Structural and stability characteristics of a monothiol glutaredoxin: Glutaredoxin-like protein 1 from Plasmodium falciparum. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1784(6), 946-952. https://doi.org/10.1016/j.bbapap.2008.03.012