Excess dietary vitamin E lowers the activities of antioxidative enzymes in erythrocytes of rats fed salmon oil - Research portal of Justus Liebig University Giessen:

Journal article

Excess dietary vitamin E lowers the activities of antioxidative enzymes in erythrocytes of rats fed salmon oil

Authors list: Eder, K; Flader, D; Hirche, F; Brandsch, C

Publication year: 2002

Pages: 3400-3404

Journal: The Journal of Nutrition

Volume number: 132

Issue number: 11

ISSN: 0022-3166

DOI Link: https://doi.org/10.1093/jn/132.11.3400

Publisher: Elsevier

Abstract:
In vitro studies suggest that high vitamin E supplementation has prooxidative activity, but very few studies have investigated this effect in vivo. We investigated the effect of excess vitamin E on the antioxidative status of rat erythrocytes and indicators of hemolysis. Six groups of growing male Sprague-Dawley rats were fed purified diets with three different vitamin E doses [100, 1000 and 10,000 mg all-rac-alpha-tocopheryl acetate (TA)/kg diet] and two different dietary fats (salmon oil and lard) for 8 wk. The rats whose diet contained salmon oil and 10,000 mg TA/kg had lower activities of superoxide dismutase (P < 0.05), glutathione peroxidase (P < 0.05), catalase (P < 0.05) and glucose-6-phosphate dehydrogenase (P < 0.05) and a lower concentration of glutathione (P < 0.05) in the erythrocyte cytosol than rats whose diet contained 100 mg TA/kg. The concentration of free hemoglobin and the binding capacity of haptoglobin in plasma, both indicators of in vivo hemolysis, did not differ between rats fed the salmon oil diet with 100 or 10,000 mg TA/kg. In the rats whose diet contained lard, the activities of antioxidant enzymes in erythrocytes and indicators of in vivo hemolysis were independent of the dietary vitamin E concentration. The results of the study suggest that an excessive vitamin E intake, when combined with salmon oil in the diet, lowers the activities of antioxidant enzymes in erythrocytes without affecting in vivo hemolysis.

Authors/Editors

- Uni.-Prof. Dr. Klaus Josef Eder

Citation Styles

Harvard Citation style: Eder, K., Flader, D., Hirche, F. and Brandsch, C. (2002) Excess dietary vitamin E lowers the activities of antioxidative enzymes in erythrocytes of rats fed salmon oil, The Journal of Nutrition, 132(11), pp. 3400-3404. https://doi.org/10.1093/jn/132.11.3400

APA Citation style: Eder, K., Flader, D., Hirche, F., & Brandsch, C. (2002). Excess dietary vitamin E lowers the activities of antioxidative enzymes in erythrocytes of rats fed salmon oil. The Journal of Nutrition. 132(11), 3400-3404. https://doi.org/10.1093/jn/132.11.3400