Journal article

Publication year: 2000

Pages: 1008-1014

Journal: Theoretical and Applied Genetics

Volume number: 101

Issue number: 7

ISSN: 0040-5752

DOI Link: https://doi.org/10.1007/s001220051574

Publisher: Springer

Abstract: 
Blackleg caused by Leptosphaeria maculans is one of the most important diseases affecting oilseed rape worldwide. Sinapis arvensis is valuable for the transfer of blackleg resistance to oilseed rape (Brassica napus) because this species contains high resistance against various aggressive isolates of the blackleg fungus. These include at least one Australian isolate which has been found to overcome resistance originating from species with the Brassica B genome, until now the major source for interspecific transfer of blackleg resistance. Backcross offspring from intergeneric crosses between Brassica napus and S. arvensis were subjected to phytopathological studies and molecular cytogenetic analysis with genomic in situ hybridisation (GISH). The BC3S progenies included fertile plants exhibiting high seedling (cotyledon) and adult plant resistance associated with the presence of an acrocentric addition chromosome from S. arvensis. In addition, some individuals with adult plant resistance but cotyledon susceptibility were observed to have a normal B. napus karyotype with no visible GISH signals, indicating possible resistant introgression lines. Phytopathological analysis of selfing progenies from 3 different highly resistant BC3 plants showed that seedling and adult plant resistance are probably conferred by different loci.

Harvard Citation style: Snowdon, R., Winter, H., Diestel, A. and Sacristan, M. (2000) Development and characterisation of Brassica napus-Sinapis arvensis addition lines exhibiting resistance to Leptosphaeria maculans, TAG Theoretical and Applied Genetics, 101(7), pp. 1008-1014. https://doi.org/10.1007/s001220051574

APA Citation style: Snowdon, R., Winter, H., Diestel, A., & Sacristan, M. (2000). Development and characterisation of Brassica napus-Sinapis arvensis addition lines exhibiting resistance to Leptosphaeria maculans. TAG Theoretical and Applied Genetics. 101(7), 1008-1014. https://doi.org/10.1007/s001220051574