Journalartikel

Jahr der Veröffentlichung: 2013

Seiten: 382-389

Zeitschrift: Plant Breeding

Bandnummer: 132

Heftnummer: 4

ISSN: 0179-9541

DOI Link: https://doi.org/10.1111/pbr.12073

Verlag: Wiley

Abstract: 
A major quantitative trait locus (QTL) influencing seed fibre and colour in Brassica napus was dissected by marker saturation in a doubled haploid (DH) population from the black-seeded oilseed rape line 'Express 617' crossed with a yellow-seeded B. napus line, '1012-98'. The marker at the peak of a sub-QTL with a strong effect on both seed colour and acid detergent lignin content lay only 4 kb away from a Brassica (H+)-ATPase gene orthologous to the transparent testa gene AHA10. Near the peak of a second sub-QTL, we mapped a copy of the key phenylpropanoid biosynthesis gene cinnamyl alcohol dehydrogenase, while another key phenylpropanoid biosynthesis gene, cinnamoyl co-a reductase 1, was found nearby. In a cross between 'Express 617' and another dark-seeded parent, 'V8', Bna.CCR1 was localized in silico near the peak of a corresponding seed fibre QTL, whereas in this case Bna. CAD2/CAD3 lay nearby. Re-sequencing of the two phenylpropanoid genes via next-generation amplicon sequencing revealed intragenic rearrangements and functionally relevant allelic variation in the three parents.

Harvard-Zitierstil: Stein, A., Wittkop, B., Liu, L., Obermeier, C., Friedt, W. and Snowdon, R. (2013) Dissection of a major QTL for seed colour and fibre content in Brassica napus reveals colocalization with candidate genes for phenylpropanoid biosynthesis and flavonoid deposition, Plant Breeding, 132(4), pp. 382-389. https://doi.org/10.1111/pbr.12073

APA-Zitierstil: Stein, A., Wittkop, B., Liu, L., Obermeier, C., Friedt, W., & Snowdon, R. (2013). Dissection of a major QTL for seed colour and fibre content in Brassica napus reveals colocalization with candidate genes for phenylpropanoid biosynthesis and flavonoid deposition. Plant Breeding. 132(4), 382-389. https://doi.org/10.1111/pbr.12073