Journal article

Genomic in situ hybridization in Brassica amphidiploids and interspecific hybrids


Authors listSnowdon, RJ; Köhler, W; Friedt, W; Köhler, A

Publication year1997

Pages1320-1324

JournalTheoretical and Applied Genetics

Volume number95

Issue number8

ISSN0040-5752

DOI Linkhttps://doi.org/10.1007/s001220050699

PublisherSpringer


Abstract
Genomic in situ hybridization (GISH) methods were used to detect different genome components within Brassica amphidiploid species and to identify donor chromatin in hybrids between Brassica napus and Raphanus sativus. In Brassica juncea and Brassica carinata the respective diploid donor genomes could be reliably distinguished by GISH, as could all R-genome chromosomes in the intergeneric hybrids. The A- and C-genome components in B. napus could not be clearly distinguished from one another using GISH, confirming the considerable homoeology between these genomes. GISH methods will be extremely beneficial for monitoring chromatin transfer and introgression in interspecific Brassica hybrids.



Citation Styles

Harvard Citation styleSnowdon, R., Köhler, W., Friedt, W. and Köhler, A. (1997) Genomic in situ hybridization in Brassica amphidiploids and interspecific hybrids, TAG Theoretical and Applied Genetics, 95(8), pp. 1320-1324. https://doi.org/10.1007/s001220050699

APA Citation styleSnowdon, R., Köhler, W., Friedt, W., & Köhler, A. (1997). Genomic in situ hybridization in Brassica amphidiploids and interspecific hybrids. TAG Theoretical and Applied Genetics. 95(8), 1320-1324. https://doi.org/10.1007/s001220050699


Last updated on 2025-21-05 at 16:11