Journal article

Publication year: 2004

Pages: 917-921

Journal: Theoretical and Applied Genetics

Volume number: 109

Issue number: 5

ISSN: 0040-5752

DOI Link: https://doi.org/10.1007/s00122-004-1713-x

Publisher: Springer

Abstract: 
Previous hybridisation studies showed that the repetitive DNA sequence pBNBH35 from Brassica nigra (genome BB, 2n=16) bound specifically to the B-genome and not to the A- or C-genomes of Brassica species. We amplified a sub-fragment of pBNBH35 from B. nigra by PCR, cloned and sequenced this sub-fragment, and confirmed that it was a 329-bp sub-fragment of pBNBH35. PCR and hybridisation techniques were used to confirm that the pBNBH35 sub-fragment was Brassica B-genome-specific. Fluorescence in situ hybridisation (FISH) in B. nigra, B. juncea (AABB, 2n=36) and B. napus (AACC, 2n=38) showed that the pBNBH35 sub-fragment was present on all eight Brassica B-genome chromosomes and absent from the A- and C-genome chromosomes. The pBNBH35 repeat was localised to the centromeric region of each B-genome chromosome. FISH clearly distinguished the B-genome chromosomes from the A-genome chromosomes in the amphidiploid species B. juncea. This is the first known report of a B-genome repetitive marker that is present on all B-genome chromosomes. It will be a useful tool for the detection of B chromosomes in interspecific hybrids and may prove useful for phylogenetic studies in Brassica species.

Harvard Citation style: Schelfhout, C., Snowdon, R., Cowling, W. and Wroth, J. (2004) A PCR based B-genome-specific marker in Brassica species, TAG Theoretical and Applied Genetics, 109(5), pp. 917-921. https://doi.org/10.1007/s00122-004-1713-x

APA Citation style: Schelfhout, C., Snowdon, R., Cowling, W., & Wroth, J. (2004). A PCR based B-genome-specific marker in Brassica species. TAG Theoretical and Applied Genetics. 109(5), 917-921. https://doi.org/10.1007/s00122-004-1713-x