Journal article

Publication year: 2016

Pages: 598-606

Journal: Plant Breeding

Volume number: 135

Issue number: 5

ISSN: 0179-9541

DOI Link: https://doi.org/10.1111/pbr.12394

Publisher: Wiley

Abstract: 
Improved cold tolerance during the juvenile phase is a major breeding goal to develop new sorghum cultivars suitable as an alternative energy crop in temperate regions. The objectives of this study were to identify marker-trait associations for cold tolerance in a sorghum diversity panel fingerprinted with 2620 single nucleotide polymorphism (SNP) markers and to detect quantitative trait loci (QTL) in two F-2:3 populations. Traits of interest were dry matter growth rate (DMGR), leaf appearance rate (LAR), chlorophyll content (SPAD) and chlorophyll fluorescence (F-v/F-m and ?(PSII)) in relation to temperature. The association panel comprised 194 genotypes, while the F-2:3 populations consisted of 80 and 92 genotypes. All populations were tested under controlled conditions in a minimum of four temperature regimes ranging from 9.4 degrees C to 20.8 degrees C. QTL were identified for means across environments and regression parameters describing temperature effects. Several marker-trait associations for mean (m) DMGR, base temperature (T-b) of SPAD and ?(PSII) and temperature effect on LAR were validated by QTL detected in population 1 or 2. Promising QTL regions were found on chromosomes SBI-01, SBI-02, SBI-03, SBI-04, SBI-06 and SBI-09, among them genomic regions where candidate genes involved in the C-repeat binding pathway or encoding cold-shock proteins are located.

Harvard Citation style: Fiedler, K., Bekele, W., Matschegewski, C., Snowdon, R., Wieckhorst, S., Zacharias, A., et al. (2016) Cold tolerance during juvenile development in sorghum: a comparative analysis by genomewide association and linkage mapping, Plant Breeding, 135(5), pp. 598-606. https://doi.org/10.1111/pbr.12394

APA Citation style: Fiedler, K., Bekele, W., Matschegewski, C., Snowdon, R., Wieckhorst, S., Zacharias, A., & Uptmoor, R. (2016). Cold tolerance during juvenile development in sorghum: a comparative analysis by genomewide association and linkage mapping. Plant Breeding. 135(5), 598-606. https://doi.org/10.1111/pbr.12394