Journalartikel

Jahr der Veröffentlichung: 2013

Seiten: 212-217

Zeitschrift: Gene

Bandnummer: 517

Heftnummer: 2

DOI Link: https://doi.org/10.1016/j.gene.2012.12.012

URL: http://europepmc.org/abstract/med/23296060

Verlag: Elsevier

Abstract: 

Invasive tephritid fruit flies are a great threat to agriculture worldwide and warrant serious pest control measures. Molecular strategies that promote embryonic lethality in these agricultural pests are limited by the small amount of nucleotide sequence data available for tephritids. To increase the dataset for sequence mining, we generated an EST database by 454 sequencing of the caribfly,Anastrepha suspensa, a model tephridd pest. This database yielded 95,803 assembled sequences with 24% identified as independent transcripts. The percentage of caribfly sequences with hits to the closely related tephritid, Rhagoletis pomonella, transcriptome was higher (28%) than to Drosophila proteins/genes (18%) in NCBI. The database contained genes specifically expressed in embryos, genes involved in the cell death, sex-determination, and RNAi pathways, and transposable elements and microsatellites. This study significantly expands the nucleotide data available for caribflies and will be a valuable resource for gene isolation and genomic studies in tephritid insects. (C) 2013 Elsevier B.V. All rights reserved.

Harvard-Zitierstil: Nirmala, X., Schetelig, M., Yu, F. and Handler, A. (2013) An EST database of the Caribbean fruit fly, Anastrepha suspensa (Diptera: Tephritidae), Gene, 517(2), pp. 212-217. https://doi.org/10.1016/j.gene.2012.12.012

APA-Zitierstil: Nirmala, X., Schetelig, M., Yu, F., & Handler, A. (2013). An EST database of the Caribbean fruit fly, Anastrepha suspensa (Diptera: Tephritidae). Gene. 517(2), 212-217. https://doi.org/10.1016/j.gene.2012.12.012