Journalartikel

Jahr der Veröffentlichung: 2014

Seiten: e109690-

Zeitschrift: PLoS ONE

Bandnummer: 9

Heftnummer: 10

Open Access Status: Gold

DOI Link: https://doi.org/10.1371/journal.pone.0109690

URL: http://europepmc.org/abstract/med/25303238

Verlag: Public Library of Science

Abstract: 

Site-specific recombination technologies are powerful new tools for the manipulation of genomic DNA in insects that can improve transgenesis strategies such as targeting transgene insertions, allowing transgene cassette exchange and DNA mobilization for transgene stabilization. However, understanding the fitness cost implications of these manipulations for transgenic strain applications is critical. In this study independent piggyBac-mediated attP target-sites marked with DsRed were created in several genornic positions in the Mexican fruit fly, Anastrepha lud ens Two of these strains, one having an autosonlal (attP_F7) and the other a Y-linked (attP_2-1V16y) integration, exhibited fitness parameters (dynamic demography and sexual competitiveness) similar to wild type flies. These strains were thus selected for targeted insertion using, for the first time in mexfly, the iahiC *ntegrase recombination system to insert an additional EGFP-marked transgene to determine its effect on host strain fitness. Fitness tests showed that the integration event in the int 2-M6y recombinant strain had no significant effect, while the int_F7 recombinant strain exhibited significantly lower fitness relative to the original attP_F7 target site host strain. These results indicate that while targeted transgene integrations can be achieved withoLit an additional fitness cost, at some genomic positions insertion of additional DNA into a previously integrated transgene can have a significant negative effect. Thus, for targeted transgene insertions fitness costs mList be evaluated both previous to and subsequent to new site-specific insertions in the target-site strain.

Harvard-Zitierstil: Meza, J., Díaz-Fleischer, F., Sánchez-Velásquez, L., Zepeda-Cisneros, C., Handler, A. and Schetelig, M. (2014) Fitness cost implications of PhiC31-mediated site-specific integrations in target-site strains of the Mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae), PLoS ONE, 9(10), p. e109690. https://doi.org/10.1371/journal.pone.0109690

APA-Zitierstil: Meza, J., Díaz-Fleischer, F., Sánchez-Velásquez, L., Zepeda-Cisneros, C., Handler, A., & Schetelig, M. (2014). Fitness cost implications of PhiC31-mediated site-specific integrations in target-site strains of the Mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae). PLoS ONE. 9(10), e109690. https://doi.org/10.1371/journal.pone.0109690