Contribution in an anthology

Appeared in: Yellow Biotechnology, II, Insect Biotechnology in Plant Protection and Industry

Editor list: Vilcinskas, Andreas

Publication year: 2013

Pages: 123-133

ISBN: 978-3-642-39901-5

eISBN: 978-3-642-39902-2

DOI Link: https://doi.org/10.1007/10_2013_209

URL: http://europepmc.org/abstract/med/23719710

Title of series: Advances in Biochemical Engineering/Biotechnology

Number in series: 136

Abstract: 

Insect pest control programs incorporating the sterile insect technique (SIT) rely on the mass production and release of sterilized insects to reduce the wild-type population through infertile matings. Most effective programs release only males to avoid any crop damage caused by female fruit flies or transmission of disease by female mosquitoes. Therefore, the females have to be eliminated, preferably in an early developmental stage, during mass rearing. Different systems and techniques have been created for the sex separation of a few insect species. One of these is the transgenic sex-specific fluorescent protein marking of the insects with automated fluorescent-based sorting of the individuals to achieve sex separation. Here we describe the Y-linked integration of fluorescent markers driven by the widely active Drosophila melanogaster polyubiquitin promoter in the Caribfly, Anastrepha suspensa. Four strains with Y-linked integrations were established with one line expressing the DsRed fluorescent protein marker during embryogenesis. This line now has the possibility for use with automated sex separation in rearing, and the same transgene markers could be used in other insects for similar applications.

Harvard Citation style: Schetelig, M. and Handler, A. (2013) Y-linked markers for improved population control of the tephritid fruit fly pest, Anastrepha suspensa, in Vilcinskas, A. (ed.) Yellow Biotechnology, II, Insect Biotechnology in Plant Protection and Industry. Heidelberg: Springer, pp. 123-133. https://doi.org/10.1007/10_2013_209

APA Citation style: Schetelig, M., & Handler, A. (2013). Y-linked markers for improved population control of the tephritid fruit fly pest, Anastrepha suspensa. In Vilcinskas, A. (Ed.), Yellow Biotechnology, II, Insect Biotechnology in Plant Protection and Industry (pp. 123-133). Springer. https://doi.org/10.1007/10_2013_209