Conference paper

Appeared in: Brassica '97

Editor list: Thomas, Grégoire; Monteiro, António A.

Publication year: 1998

Pages: 127-134

ISBN: 90-6605-820-X

DOI Link: https://doi.org/10.17660/ActaHortic.1998.459.13

Conference: International Symposium on Brassicas

Title of series: Acta horticulturae

Number in series: 459

Abstract: 

Fluorescence in situ hybridization (FISH) methods were developed for Brassica which allow the physical localization of labeled DNA sequences at chromosomal sub-arm level. In addition, genomic in situ hybridization (GISH) enabled all Brassica diploid genome components apart from the highly similar A and C genomes to be distinguished, and R genome chromatin was reliably detected in Brassica napus x Raphanus sativus hybrids. Hybrids among the Brassicaceae can be relatively easily produced and are an ideal method for generating new lines containing agronomically important pest and disease resistance genes. Efficient monitoring of alien chromatin is however critical for successful transfer of alien chromosomes or chromosome segments containing useful genes. The small size of chromosomes in Brassica and its close relatives is particularly problematic for the identification of alien chromosomes in Brassica hybrids, and translocations cannot be reliably identified by conventional cytogenetic methods. GISH was shown to be an effective practical method for detecting amounts and locations of foreign chromatin in Brassica hybrids, while other FISH applications were useful for chromosome identification and investigations of Brassica genome evolution.

Harvard Citation style: Snowdon, R., Köhler, W., Köhler, A. and Friedt, W. (1998) Fluorescence in situ hybridization techniques for Brassica: Methodological development and practical applications, in Thomas, G. and Monteiro, A. (eds.) Brassica '97. Leuven: International Society for Horticultural Science. pp. 127-134. https://doi.org/10.17660/ActaHortic.1998.459.13

APA Citation style: Snowdon, R., Köhler, W., Köhler, A., & Friedt, W. (1998). Fluorescence in situ hybridization techniques for Brassica: Methodological development and practical applications. In Thomas, G., & Monteiro, A. (Eds.), Brassica '97. (pp. 127-134). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.1998.459.13