Journal article

Publication year: 2004

Pages: 91-100

Journal: Plant Science: An international journal of experimental plant biology

Volume number: 167

Issue number: 1

ISSN: 0168-9452

DOI Link: https://doi.org/10.1016/S0168-9452(04)00111-6

Publisher: Elsevier

Abstract: 
The biochemical reaction of maize (Zea mays L.) to salt stress at the level of proteins in roots and shoots is shown here for the first time. Maize is considered as a salt-sensitive plant. We used an Na+-excluding maize inbred line and low NaCl concentrations to minimize ion effects. Protein patterns were analyzed using 2D polyacrylamide gel electrophoresis. High, as well as low, NaCl treatment of maize led to an unexpected high number of differentially regulated proteins in roots and shoots. Moderate salt stress (25 mM NaCl) already led to a differential regulation of 31% of shoot proteins and 45% of root proteins, without an effect on the morphology and the Na+ and Cl- concentrations of the plants. High stress (100 mM NaCl) led to an uncontrolled change of more than 80% of the separated proteins. Fourteen proteins which were increased by salt stress were identified by in-gel digestion and peptide mass fingerprinting using a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF). We detected three groups of differentially regulated proteins under low salts stress. (A) proteins which are involved in protein biosynthesis and protein modifications by kinases, (13) enzymes of carbon metabolism, and (C) enzymes of the nitrogen metabolism. Eight of these 14 proteins have been reported in the literature to be differentially regulated under high NaCl stress at the level of transcription, translation or metabolism. According to our data there appears to be no specific adaptation to salt stress in maize at the level of proteins. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

Harvard Citation style: Zörb, C., Schmitt, S., Neeb, A., Karl, S., Linder, M. and Schubert, S. (2004) The biochemical reaction of maize (Zea mays L.) to salt stress is characterized by a mitigation of symptoms and not by a specific adaptation, Plant Science: An international journal of experimental plant biology, 167(1), pp. 91-100. https://doi.org/10.1016/S0168-9452(04)00111-6

APA Citation style: Zörb, C., Schmitt, S., Neeb, A., Karl, S., Linder, M., & Schubert, S. (2004). The biochemical reaction of maize (Zea mays L.) to salt stress is characterized by a mitigation of symptoms and not by a specific adaptation. Plant Science: An international journal of experimental plant biology. 167(1), 91-100. https://doi.org/10.1016/S0168-9452(04)00111-6