Journal article

Publication year: 2011

Pages: 941-949

Journal: Free Radical Research

Volume number: 45

Issue number: 8

ISSN: 1071-5762

DOI Link: https://doi.org/10.3109/10715762.2011.571683

Publisher: Taylor and Francis Group

Abstract: 
There is increasing interest in the gene-regulatory activity of Brassica vegetable derived phytochemicals such as 3,3'-diindolylmethane (DIM) and indole-3-carbinol (I3C). DIM is formed under acidic conditions by dimerization of I3C. This study compared the Nrf2 activating potential of DIM and I3C in murine fibroblasts (NIH3T3). In contrast to its precursor I3C, DIM induces the transactivation of Nrf2. Furthermore, Nrf2 targets such as HO-1, gamma GCS and NQO1 were increased on the mRNA and protein levels following DIM treatment. DIM was less potent than sulforaphane (used as positive control) in inducing Nrf2-dependent gene expression. The present data suggest that the dimerization of I3C to DIM increases its Nrf2 inducing activity.

Harvard Citation style: Ernst, I., Schuemann, C., Wagner, A. and Rimbach, G. (2011) 3,3 '-Diindolylmethane but not indole-3-carbinol activates Nrf2 and induces Nrf2 target gene expression in cultured murine fibroblasts, Free Radical Research, 45(8), pp. 941-949. https://doi.org/10.3109/10715762.2011.571683

APA Citation style: Ernst, I., Schuemann, C., Wagner, A., & Rimbach, G. (2011). 3,3 '-Diindolylmethane but not indole-3-carbinol activates Nrf2 and induces Nrf2 target gene expression in cultured murine fibroblasts. Free Radical Research. 45(8), 941-949. https://doi.org/10.3109/10715762.2011.571683