Allyl isothiocyanate as a potential inducer of paraoxonase-1-studies in cultured hepatocytes and in mice - Research portal of Justus Liebig University Giessen:

Journal article

Allyl isothiocyanate as a potential inducer of paraoxonase-1-studies in cultured hepatocytes and in mice

Authors list: Schrader, C; Graeser, AC; Huebbe, P; Wagner, AE; Rimbach, G

Publication year: 2012

Pages: 162-168

Journal: IUBMB Life

Volume number: 64

Issue number: 2

ISSN: 1521-6543

DOI Link: https://doi.org/10.1002/iub.587

Publisher: Wiley

Abstract:
In this study, we tested the ability of structure-related isothiocyanates to induce the antiatherogenic enzyme paraoxonase-1 (PON1) in cultured hepatocytes. Allyl isothiocyanate (AITC), phenylethyl isothiocyanate (PEITC), and sulforaphane (SFN), but not butyl isothiocyanate (BITC) resulted in dose-dependent induction of PON1 transactivation in Huh7 cells in vitro. Induction of PON1 due to AITC was inhibited by the selective peroxisome proliferator-activated receptor gamma-antagonist T0070907. AITC was used in a subsequent in vivo study in mice (n = 10 per group, Western-type diet) to test its PON1 inducing activity. Unlike in cultured hepatocytes, AITC supplementation (15 mg/kg body weight) did not increase hepatic PON1 mRNA and protein levels in mice. Thus, it is suggested that AITC may be a potent inducer of PON1 in vitro, but not in mouse liver in vivo. (c) 2011 IUBMB, IUBMB Life,, 2011.

Authors/Editors

- Uni.-Prof. Dr. Anika Eva Wagner

Citation Styles

Harvard Citation style: Schrader, C., Graeser, A., Huebbe, P., Wagner, A. and Rimbach, G. (2012) Allyl isothiocyanate as a potential inducer of paraoxonase-1-studies in cultured hepatocytes and in mice, IUBMB Life, 64(2), pp. 162-168. https://doi.org/10.1002/iub.587

APA Citation style: Schrader, C., Graeser, A., Huebbe, P., Wagner, A., & Rimbach, G. (2012). Allyl isothiocyanate as a potential inducer of paraoxonase-1-studies in cultured hepatocytes and in mice. IUBMB Life. 64(2), 162-168. https://doi.org/10.1002/iub.587