Cellular uptake, stability, visualization by 'Naturstoff reagent A', and multidrug resistance protein 1 gene-regulatory activity of cyanidin in human keratinocytes - Research portal of Justus Liebig University Giessen:

Journal article

Cellular uptake, stability, visualization by 'Naturstoff reagent A', and multidrug resistance protein 1 gene-regulatory activity of cyanidin in human keratinocytes

Authors list: Ernst, IMA; Wagner, AE; Lipinski, S; Skrbek, S; Ruefer, CE; Desel, C; Rimbach, G

Publication year: 2010

Pages: 253-258

Journal: Pharmacological Research

Volume number: 61

Issue number: 3

ISSN: 1043-6618

DOI Link: https://doi.org/10.1016/j.phrs.2009.10.006

Publisher: Elsevier

Abstract:
There is increasing interest in the role of anthocyanidins as potential skin protective phytochemicals. However, little is known if and to what extent anthocyanidins are taken up by the human skin. In the present study cellular uptake (as determined by HPLC), stability, and gene-regulatory activity of cyanidin were determined in human HaCaT keratinocytes in culture. Using the fluorescent dye Naturstoff reagent A cyanidin was visualized in order to determine its cellular accumulation via flow cytometry and fluorescence microscopy.Cyanidin was rapidly taken up by HaCaT cells at relatively low concentrations. Following incubation, cellular cyanidin levels decreased time-dependently most likely due to degradation into protocatechuic acid and phloroglucinol aldehyde. Confocal laser scanning microscopy data demonstrated that cyanidin was mainly present in the cytoplasm. Cellular uptake of cyanidin was accompanied by an inhibition of multidrug resistance protein 1 (involved in cellular efflux of flavonoids) mRNA-levels indicating its gene-regulatory activity.Naturstoff reagent A seems to be a promising fluorescent dye to visualize cyanidin in keratinocytes. (C) 2009 Elsevier Ltd. All rights reserved.

Authors/Editors

- Uni.-Prof. Dr. Anika Eva Wagner

Citation Styles

Harvard Citation style: Ernst, I., Wagner, A., Lipinski, S., Skrbek, S., Ruefer, C., Desel, C., et al. (2010) Cellular uptake, stability, visualization by 'Naturstoff reagent A', and multidrug resistance protein 1 gene-regulatory activity of cyanidin in human keratinocytes, Pharmacological Research, 61(3), pp. 253-258. https://doi.org/10.1016/j.phrs.2009.10.006

APA Citation style: Ernst, I., Wagner, A., Lipinski, S., Skrbek, S., Ruefer, C., Desel, C., & Rimbach, G. (2010). Cellular uptake, stability, visualization by 'Naturstoff reagent A', and multidrug resistance protein 1 gene-regulatory activity of cyanidin in human keratinocytes. Pharmacological Research. 61(3), 253-258. https://doi.org/10.1016/j.phrs.2009.10.006