Journal article

Publication year: 2007

Pages: 319-324

Journal: Biochemical and Biophysical Research Communications

Volume number: 357

Issue number: 1

ISSN: 0006-291X

DOI Link: https://doi.org/10.1016/j.bbrc.2007.03.150

Publisher: Elsevier

Abstract: 
In order to gain a more comprehensive understanding of the aetiology of apolipoprotein E4 genotype-cardiovascular disease (CVD) associations, the impact of the apoE genotype on the macrophage inflammatory response was examined. The murine monocyte-macrophage cell line (RAW 264.7) stably transfected to produce equal amounts of human apoE3 or apoE4 was used. Following LPS stimulation, apoE4-macrophages showed higher and lower concentrations of tumour necrosis factor alpha (pro-inflammatory) and interleukin 10 (anti-inflammatory), respectively, both at mRNA and protein levels. In addition, increased expression of heme oxygenase-1 (a stress-induced anti-inflammatory protein) was observed in the apoE4-cells. Furthermore, in apoE4-macrophages, an enhanced transactivation of the key redox sensitive transcription factor NF-kappa B was shown. Current data indicate that apoE4 macrophages have an altered inflammatory response, which may contribute to the higher CVD risk observed in apoE4 carriers. (c) 2007 Elsevier Inc. All rights reserved.

Harvard Citation style: Jofro-Monseny, L., Loboda, A., Wagner, A., Huebbe, P., Boesch-Saadatmandi, C., Jozkowicz, A., et al. (2007) Effects of apoE genotype on macrophage inflammation and heme oxygenase-1 expression, Biochemical and Biophysical Research Communications, 357(1), pp. 319-324. https://doi.org/10.1016/j.bbrc.2007.03.150

APA Citation style: Jofro-Monseny, L., Loboda, A., Wagner, A., Huebbe, P., Boesch-Saadatmandi, C., Jozkowicz, A., Minihane, A., Dulak, J., & Rimbach, G. (2007). Effects of apoE genotype on macrophage inflammation and heme oxygenase-1 expression. Biochemical and Biophysical Research Communications. 357(1), 319-324. https://doi.org/10.1016/j.bbrc.2007.03.150