Epigallocatechin gallate affects glucose metabolism and increases fitness and lifespan in Drosophila melanogaster - Research portal of Justus Liebig University Giessen:

Journal article

Epigallocatechin gallate affects glucose metabolism and increases fitness and lifespan in Drosophila melanogaster

Authors list: Wagner, AE; Piegholdt, S; Rabe, D; Baenas, N; Schloesser, A; Eggersdorfer, M; Stocker, A; Rimbach, G

Publication year: 2015

Pages: 30568-30578

Journal: Oncotarget

Volume number: 6

Issue number: 31

DOI Link: https://doi.org/10.18632/oncotarget.5215

Publisher: Impact Journals

Abstract:
In this study, we tested whether a standardized epigallocatechin-3-gallate (EGCG) rich green tea extract (comprising > 90% EGCG) affects fitness and lifespan as well as parameters of glucose metabolism and energy homeostasis in the fruit fly, Drosophila melanogaster. Following the application of the green tea extract a significant increase in the mean lifespan (+ 3.3 days) and the 50% survival (+ 4.3 days) as well as improved fitness was detected. These effects went along an increased expression of Spargel, the homolog of mammalian PGC1a, which has been reported to affect lifespan in flies. Intriguingly, in flies, treatment with the green tea extract decreased glucose concentrations, which were accompanied by an inhibition of alpha-amylase and alpha-glucosidase activity. Computational docking analysis proved the potential of EGCG to dock into the substrate binding pocket of alpha-amylase and to a greater extent into a-glucosidase. Furthermore, we demonstrate that EGCG downregulates insulin-like peptide 5 and phosphoenolpyruvate carboxykinase, major regulators of glucose metabolism, as well as the Drosophila homolog of leptin, unpaired 2. We propose that a decrease in glucose metabolism in connection with an upregulated expression of Spargel contribute to the better fitness and the extended lifespan in EGCG-treated flies.

Authors/Editors

- Uni.-Prof. Dr. Anika Eva Wagner

Citation Styles

Harvard Citation style: Wagner, A., Piegholdt, S., Rabe, D., Baenas, N., Schloesser, A., Eggersdorfer, M., et al. (2015) Epigallocatechin gallate affects glucose metabolism and increases fitness and lifespan in Drosophila melanogaster, Oncotarget, 6(31), pp. 30568-30578. https://doi.org/10.18632/oncotarget.5215

APA Citation style: Wagner, A., Piegholdt, S., Rabe, D., Baenas, N., Schloesser, A., Eggersdorfer, M., Stocker, A., & Rimbach, G. (2015). Epigallocatechin gallate affects glucose metabolism and increases fitness and lifespan in Drosophila melanogaster. Oncotarget. 6(31), 30568-30578. https://doi.org/10.18632/oncotarget.5215