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Journal article

Free radical scavenging and antioxidant activity of betanin: Electron spin resonance spectroscopy studies and studies in cultured cells

Authors list: Esatbeyoglu, T; Wagner, AE; Motafakkerazad, R; Nakajima, Y; Matsugo, S; Rimbach, G

Publication year: 2014

Pages: 119-126

Journal: Food and Chemical Toxicology

Volume number: 73

ISSN: 0278-6915

DOI Link: https://doi.org/10.1016/j.fct.2014.08.007

Publisher: Elsevier

Abstract:
Betanin is a red pigment present in red beetroot. Recently, potential health benefits of betanin-rich beetroot have been suggested. However, little is known regarding the free radical scavenging and antioxidant activity of betanin. Electron spin resonance spectroscopy (ESR) and spin trapping techniques were applied to evaluate the ability of betanin to scavenge hydroxyl, superoxide, 2,2 diphenyl-1-picrylhydrazyl (DPPH), and galvinoxyl free radicals. In addition, we tested in cultured cells the ability of betanin to prevent DNA damage and to induce the transcription factor Nrf2 (nuclear factor (erythroid-derived 2)-like 2) as well as its down-stream target heme oxygenase 1 (HO-1), paraoxonase 1 (PON1) and glutathione (GSH). Betanin dose-dependently scavenged DPPH-, galvinoxyl-, superoxide-, and hydroxyl-radicals in the ESR and spin trapping studies and prevented hydrogen peroxide induced DNA damage as determined by the Comet assay.Furthermore, betanin treatment induced the transcription factor Nrf2 and resulted in an increase of HO-1 protein levels, PON1-transactivation and cellular GSH. Present data suggest that betanin is both a free radical scavenger and an inducer of antioxidant defense mechanism in cultured cells. (C) 2014 Elsevier Ltd. All rights reserved.

Authors/Editors

- Uni.-Prof. Dr. Anika Eva Wagner

Citation Styles

Harvard Citation style: Esatbeyoglu, T., Wagner, A., Motafakkerazad, R., Nakajima, Y., Matsugo, S. and Rimbach, G. (2014) Free radical scavenging and antioxidant activity of betanin: Electron spin resonance spectroscopy studies and studies in cultured cells, Food and Chemical Toxicology, 73, pp. 119-126. https://doi.org/10.1016/j.fct.2014.08.007

APA Citation style: Esatbeyoglu, T., Wagner, A., Motafakkerazad, R., Nakajima, Y., Matsugo, S., & Rimbach, G. (2014). Free radical scavenging and antioxidant activity of betanin: Electron spin resonance spectroscopy studies and studies in cultured cells. Food and Chemical Toxicology. 73, 119-126. https://doi.org/10.1016/j.fct.2014.08.007