Journal article

Publication year: 2015

Pages: 661-666

Journal: The Journal of Nutritional Biochemistry

Volume number: 26

Issue number: 6

ISSN: 0955-2863

DOI Link: https://doi.org/10.1016/j.jnutbio.2015.01.004

Publisher: Elsevier

Abstract: 
In this study, the effect of myrosinase-treated glucoerucin (GER+MYR), which releases the isothiocyanate (ITC) erucin, on heme oxygenase 1 (HO-1) gene expression and Nrf2 signaling was investigated in vitro in cultured cells and in vivo in mice. Treatment of HT-29 cells with GER+MYR resulted in a significant increase in the mRNA and protein levels of nuclear Nrf2 and HO-1. GER+MYR was more potent at enhancing the nuclear Nrf2 levels than were the following myrosinase-treated glucosinolates: sinigrin, glucoraphanin and gluconasturtiin, which are the precursors of allyl-ITC, R-sulforaphane and 2-phenylethyl ITC, respectively. GER+MYR also significantly induced HO-1 gene expression in the mouse intestinal mucosae and liver but not in the brain. Mechanistic studies suggest that GER+MYR induces Nrf2 via ERK1/2-, p38- and JNK-dependent signal transduction pathways. The GER+MYR-mediated increase in HO-1 expression is primarily attributable to p38 signaling. (C) 2015 Elsevier Inc. All rights reserved.

Harvard Citation style: Wagner, A., Sturm, C., Piegholdt, S., Wolf, I., Esatbeyoglu, T., De Nicola, G., et al. (2015) Myrosinase-treated glucoerucin is a potent inducer of the Nrf2 target gene heme oxygenase 1-studies in cultured HT-29 cells and mice, The Journal of Nutritional Biochemistry, 26(6), pp. 661-666. https://doi.org/10.1016/j.jnutbio.2015.01.004

APA Citation style: Wagner, A., Sturm, C., Piegholdt, S., Wolf, I., Esatbeyoglu, T., De Nicola, G., Iori, R., & Rimbach, G. (2015). Myrosinase-treated glucoerucin is a potent inducer of the Nrf2 target gene heme oxygenase 1-studies in cultured HT-29 cells and mice. The Journal of Nutritional Biochemistry. 26(6), 661-666. https://doi.org/10.1016/j.jnutbio.2015.01.004