The Natural Compound Ascorbigen Modulates NADPH-Quinone Oxidoreductase (NQO1) mRNA and Enzyme Activity Levels in Cultured Liver Cells and in Laboratory Rats - Research portal of Justus Liebig University Giessen:

Journal article

The Natural Compound Ascorbigen Modulates NADPH-Quinone Oxidoreductase (NQO1) mRNA and Enzyme Activity Levels in Cultured Liver Cells and in Laboratory Rats

Authors list: Wagner, AE; Hug, H; Gössl, R; Riss, G; Mussler, B; Elste, V; Rimbach, G; Barella, L

Publication year: 2008

Pages: 122-128

Journal: Annals of Nutrition and Metabolism

Volume number: 53

Issue number: 2

ISSN: 0250-6807

DOI Link: https://doi.org/10.1159/000170887

Publisher: Karger Publishers

Abstract:
Ascorbigen (ABG) is a natural compound that represents a breakdown product of the glucosinolates that are present in Brassica vegetables. It is postulated that ABG may have anti-carcinogenic activity; however, the underlying molecular and cellular mechanisms are largely unknown. In the present study we investigated the effect of ABG on the mRNA and enzyme activity levels of NADPH-quinone oxidoreductase (NQO1), which is centrally involved in the detoxification of xenobiotics, in cultured liver cells and in rats. The mRNA levels of NQO1 showed an increase of up to 100% in cultured liver cells (HepG2) following incubation with different concentrations of ABG (3-100 mu mol/l) compared to control cells. Furthermore, NQO1 activity was elevated (up to 20%) by ABG treatment. The in vitro results were confirmed in rats who received either 5 mg/day ABG or vehicle for 7 days. Significantly higher mRNA (a 90% increase) and enzyme activity levels (a 40% increase) of NQO1 were detected in the liver of ABG-treated rats as compared to control animals. Current data indicate that ABG is a moderate inducer of the phase II enzyme NQO1, both in cultured hepatocytes and in vivo. Copyright (C) 2008 S. Karger AG, Basel

Authors/Editors

- Uni.-Prof. Dr. Anika Eva Wagner

Citation Styles

Harvard Citation style: Wagner, A., Hug, H., Gössl, R., Riss, G., Mussler, B., Elste, V., et al. (2008) The Natural Compound Ascorbigen Modulates NADPH-Quinone Oxidoreductase (NQO1) mRNA and Enzyme Activity Levels in Cultured Liver Cells and in Laboratory Rats, Annals of Nutrition and Metabolism, 53(2), pp. 122-128. https://doi.org/10.1159/000170887

APA Citation style: Wagner, A., Hug, H., Gössl, R., Riss, G., Mussler, B., Elste, V., Rimbach, G., & Barella, L. (2008). The Natural Compound Ascorbigen Modulates NADPH-Quinone Oxidoreductase (NQO1) mRNA and Enzyme Activity Levels in Cultured Liver Cells and in Laboratory Rats. Annals of Nutrition and Metabolism. 53(2), 122-128. https://doi.org/10.1159/000170887