Journalartikel

Jahr der Veröffentlichung: 2014

Seiten: 950-956

Zeitschrift: Antimicrobial Agents and Chemotherapy

Bandnummer: 58

Heftnummer: 2

ISSN: 0066-4804

DOI Link: https://doi.org/10.1128/AAC.01894-13

Verlag: American Society for Microbiology

Abstract: 
Corallopyronin A is a promising in vivo active antibiotic, currently undergoing preclinical evaluation. This myxobacterial compound interferes with a newly identified drug target site, i.e., the switch region of the bacterial DNA-dependent RNA-polymerase (RNAP). Since this target site differs from that of known RNAP inhibitors such as the rifamycins, corallopyronin A shows no cross-resistance with other antibacterial agents. Corallopyronin A is a polyketide synthase-and nonribosomal peptide synthetase- derived molecule whose structure and biosynthesis is distinguished by several peculiarities, such as the unusual vinyl carbamate functionality whose formation involves carbonic acid as an unprecedented C1-starter unit. Using in vitro experiments the nature of this starter molecule was revealed to be the methyl ester of carbonic acid. Biochemical investigations showed that methylation of carbonic acid is performed by the O-methyltransferase CorH. These experiments shed light on the biosynthesis of the Eastern chain of alpha-pyrone antibiotics such as corallopyronin A.

Harvard-Zitierstil: Schäberle, T., Mohseni, M., Lohr, F., Schmitz, A. and König, G. (2014) Function of the Loading Module in CorI and of the O-Methyltransferase CorH in Vinyl Carbamate Biosynthesis of the Antibiotic Corallopyronin A, Antimicrobial Agents and Chemotherapy, 58(2), pp. 950-956. https://doi.org/10.1128/AAC.01894-13

APA-Zitierstil: Schäberle, T., Mohseni, M., Lohr, F., Schmitz, A., & König, G. (2014). Function of the Loading Module in CorI and of the O-Methyltransferase CorH in Vinyl Carbamate Biosynthesis of the Antibiotic Corallopyronin A. Antimicrobial Agents and Chemotherapy. 58(2), 950-956. https://doi.org/10.1128/AAC.01894-13