Journalartikel

Jahr der Veröffentlichung: 2013

Seiten: 38-40

Zeitschrift: Analytical Biochemistry

Bandnummer: 432

Heftnummer: 1

ISSN: 0003-2697

DOI Link: https://doi.org/10.1016/j.ab.2012.09.026

Verlag: Elsevier

Abstract: 
Methyltransferases (MTs) catalyze the transfer of a methyl group from S-adenosylmethionine (SAM) to a suitable substrate. Such methylations are important modifications in secondary metabolisms, especially on natural products produced by polyketide synthases and nonribosomal peptide synthetases, many of which are of special interest due to their prominent pharmacological activities (e.g., lovastatin, cyclosporin). To gain basic biochemical knowledge on the methylation process, it is of immense relevance to simplify methods concerning experimental problems caused by a large variety in substrates. Here, we present a photometric method to analyze MT activity by measuring SAM consumption in a coupled enzyme assay. (C) 2012 Elsevier Inc. All rights reserved.

Harvard-Zitierstil: Schäberle, T., Siba, C., Höver, T. and König, G. (2013) An easy-to-perform photometric assay for methyltransferase activity measurements, Analytical Biochemistry, 432(1), pp. 38-40. https://doi.org/10.1016/j.ab.2012.09.026

APA-Zitierstil: Schäberle, T., Siba, C., Höver, T., & König, G. (2013). An easy-to-perform photometric assay for methyltransferase activity measurements. Analytical Biochemistry. 432(1), 38-40. https://doi.org/10.1016/j.ab.2012.09.026