Journal article

Publication year: 2010

Pages: 1253-1265

Journal: ChemBioChem

Volume number: 11

Issue number: 9

ISSN: 1439-4227

DOI Link: https://doi.org/10.1002/cbic.201000085

Publisher: Wiley

Abstract: 
Corallopyronin A is a myxobacterial compound with potent antibacterial Feeding experiments with labelled precursors the deduction all biosynthetic building blocks for corallopyronin A and revealed an unusual feature of this metabolite: tits-biosynthesis from two chains, one solely PKS-derived and the other NRPS/PKS-derived. The starter molecule is believed to be carbonic acid or its monomethyl ester, The putative corallopyronin A biosynthetic gene cluster is a trans-AT-type mixed PKS/NRPS gene cluster, containing a beta-branching cassette. Striking features of this gene cluster are a NRPS-like adenylation domain, that, is part of a PKS-type module and is believed to be responsible for glycine incorporation, as well as split modules with individual domains occurring on different genes. It is suggested that CorB is a transacting ketosynthase and it is proposed that it catalyses the Claisen condensation responsible for the interconnection of the two chains. Additionally, the stereochemistry of corallopyronin A was deduced by a combination of a modified Mosher's method and ozonolysis with subsequent chiral GC analyses.

Harvard Citation style: Erol, O., Schäberle, T., Schmitz, A., Rachid, S., Gurgui, C., El Omari, M., et al. (2010) Biosynthesis of the Myxobacterial Antibiotic Corallopyronin A, ChemBioChem, 11(9), pp. 1253-1265. https://doi.org/10.1002/cbic.201000085

APA Citation style: Erol, O., Schäberle, T., Schmitz, A., Rachid, S., Gurgui, C., El Omari, M., Lohr, F., Kehraus, S., Piel, J., Muller, R., & König, G. (2010). Biosynthesis of the Myxobacterial Antibiotic Corallopyronin A. ChemBioChem. 11(9), 1253-1265. https://doi.org/10.1002/cbic.201000085