Journalartikel

Jahr der Veröffentlichung: 2018

Seiten: 79-86

Zeitschrift: JPC – Journal of Planar Chromatography – Modern TLC

Bandnummer: 31

Heftnummer: 1

DOI Link: https://doi.org/10.1556/1006.2018.31.1.10

Verlag: Akadémiai Kiadó

Abstract: 

A method was developed for effect-directed analysis (EDA) of the root extract of Pimpinella saxifraga L. High-performance thin-layer chromatography (HPTLC) was hyphenated with microchemical, biochemical, and biological assays as well as electrospray ionization-mass spectrometry (ESI-MS). This HPTLC-UV/Vis/FLDEDA-MS method directly pointed to multi-potent compounds in the P. saxifraga L. root extract. 2,2-Diphenyl-1-picrylhydrazyl radical scavengers, acetylcholinesterase inhibitors, estrogen-effective compounds, antimicrobials against Gram-positive Bacillus subtilis bacteria, and Gram-negative Aliivibrio fischeri bacteria were discovered in the root extract. A first targeted characterization of four unknown multi-potent compounds was performed by HPTLC-ESI-MS and microchemical derivatizations. This highly streamlined effect-directed profiling is recommended for a fast and cost-efficient natural product search.

Harvard-Zitierstil: Morlock, G. and Lapin, T. (2018) Effect-directed analysis of Pimpinella saxifraga L. root extract via HPTLC–UV/Vis/FLD–EDA–MS, JPC – Journal of Planar Chromatography – Modern TLC, 31(1), pp. 79-86. https://doi.org/10.1556/1006.2018.31.1.10

APA-Zitierstil: Morlock, G., & Lapin, T. (2018). Effect-directed analysis of Pimpinella saxifraga L. root extract via HPTLC–UV/Vis/FLD–EDA–MS. JPC – Journal of Planar Chromatography – Modern TLC. 31(1), 79-86. https://doi.org/10.1556/1006.2018.31.1.10