Journal article

Effect-directed analysis via hyphenated high-performance thin-layer chromatography for bioanalytical profiling of sunflower leaves


Authors list: Móricz, A.M.; Ott, P.G.; Yüce, I.; Darcsi, A.; Béni, S.; Morlock, G.E.

Publication year: 2018

Pages: 213-220

Journal: Journal of Chromatography A

Volume number: 1533

DOI Link: https://doi.org/10.1016/j.chroma.2017.12.034

Publisher: Elsevier


Abstract: 

High-performance thin-layer chromatography (HPTLC) coupled with effect-directed analysis was used for non-targeted screening of sunflower leaf extract for components exhibiting antioxidant, antibacterial and/or cholinesterase enzyme inhibitory effects. The active compounds were characterized by HPTLC-electrospray ionization-high resolution mass spectrometry (ESI-HRMS) and HPTLC-Direct Analysis in Real Time (DART)-MS/MS. The latter ambient ionization technique (less soft than ESI) resulted in oxidation and fragmentation products and characteristic fragment ions. NMR spectroscopy after targeted isolation via preparative normal phase flash chromatography and semi-preparative reversed phase high-performance liquid chromatography supported the identification of two diterpenes to be (-)-kaur-16-en-19-oic acid and 15-alpha-angeloyloxy-ent-kaur-16-en-19-oic acid. Both compounds found to be multi-potent as they inhibited acetylcholinesterase and butyrylcholinesterase and showed antibacterial effects against Gram-positive Bacillus subtilis and Gram-negative Aliivibrio fischeri bacteria. Kaurenoic acid was also active against the Gram-negative pepper pathogenic Xanthomonas euvesicatoria bacteria. (C) 2017 Elsevier B.V. All rights reserved.




Citation Styles

Harvard Citation style: Móricz, A., Ott, P., Yüce, I., Darcsi, A., Béni, S. and Morlock, G. (2018) Effect-directed analysis via hyphenated high-performance thin-layer chromatography for bioanalytical profiling of sunflower leaves, Journal of Chromatography A, 1533, pp. 213-220. https://doi.org/10.1016/j.chroma.2017.12.034

APA Citation style: Móricz, A., Ott, P., Yüce, I., Darcsi, A., Béni, S., & Morlock, G. (2018). Effect-directed analysis via hyphenated high-performance thin-layer chromatography for bioanalytical profiling of sunflower leaves. Journal of Chromatography A. 1533, 213-220. https://doi.org/10.1016/j.chroma.2017.12.034


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