Journal article

Effect-directed discovery of bioactive compounds followed by highly targeted characterization, isolation and identification, exemplarily shown for solidago virgaurea


Authors list: Móricz, A.M.; Ott, P.G.; Häbe, T.T.; Darcsi, A.; Böszörményi, A.; Alberti, A.; Krüzselyi, D.; Csontos, P.; Béni, S.; Morlock, G.E.

Publication year: 2016

Pages: 8202-8209

Journal: Analytical Chemistry

Volume number: 88

Issue number: 16

DOI Link: https://doi.org/10.1021/acs.analchem.6b02007

Publisher: American Chemical Society


Abstract: 

A nontargeted; effect-directed screening (bioprofiling) and a subsequent highly targeted characterization of antibacterial compounds from plant matrices is demonstrated on the example of Solidago virgaurea root extracts. The procedure comprises high-performance thin-layer chromatography (HPTLC) coupled with six bacterial bioassays including two plant pathogens, a radical scavenging assay, an acetylcholinesterase assay as well as in situ and ex situ mass spectrometric analyses. In situ mass spectra were directly recorded from the adsorbent using the Direct Analysis in Real Time interface (HPTLC-DARTMS), whereas ex situ mass spectra were recorded using an elution head-based interface (HPTLC-ESI-MS): For further bioassay guided isolation of the main antimicrobial compounds, flash chromatographic fractionation and semipreparative highperformance liquid chromatographic purification were used and nuclear magnetic resonance data allowed the identification of the unknown antimicrobial compounds as 2Z,8Z- and 2E,8Z-matricaria esters. The discovered antibacterial activity was confirmed and specified by a luminometric assay and, as minimal inhibitory concentration in the liquid phase.




Citation Styles

Harvard Citation style: Móricz, A., Ott, P., Häbe, T., Darcsi, A., Böszörményi, A., Alberti, A., et al. (2016) Effect-directed discovery of bioactive compounds followed by highly targeted characterization, isolation and identification, exemplarily shown for solidago virgaurea, Analytical Chemistry, 88(16), pp. 8202-8209. https://doi.org/10.1021/acs.analchem.6b02007

APA Citation style: Móricz, A., Ott, P., Häbe, T., Darcsi, A., Böszörményi, A., Alberti, A., Krüzselyi, D., Csontos, P., Béni, S., & Morlock, G. (2016). Effect-directed discovery of bioactive compounds followed by highly targeted characterization, isolation and identification, exemplarily shown for solidago virgaurea. Analytical Chemistry. 88(16), 8202-8209. https://doi.org/10.1021/acs.analchem.6b02007


Last updated on 2025-21-05 at 16:20