Journalartikel

Jahr der Veröffentlichung: 2015

Seiten: 11098-11104

Zeitschrift: Analytical Chemistry

Bandnummer: 87

Heftnummer: 21

DOI Link: https://doi.org/10.1021/acs.analchem.5b03233

Verlag: American Chemical Society

Abstract: 

A direct bioautography has been used for the simultaneous determination of four estrogens [estrone (El), 17 beta-estradiol (E2), estriol (E3), and 17 alpha-ethinylestradiol (EE2)] and two xenoestrogens [bisphenol A (BPA) and 4-n-nonyl-phenol (NP)] in surface water and wastewater samples from a sewage treatment plant. After either direct application or a liquid liquid extraction of the water samples, the qualitative and quantitative detection of estrogen-effective compounds was performed with a planar yeast estrogen screen. The limits of detection were different for each compound, due to the specific receptor binding of individual (xeno)estrogens (1 ng/L to 15 mu g/L). The mean recovery rate for all six substances at this ultratrace level was 88% [mean percent relative standard deviation (%RSD) of 17%, n = 3]. Over the whole procedure, precisions of three estrogens discovered sample were below 17%, n = 3. The identification of the detected bioactive compounds was performed by high-performance thin-layer chromatography-electrospray ionization mass spectrometry (HPTLC-ESI-MS) via the elution-head-based TLC-MS Interface. Whereas the estrogens E1 and E2 could always be detected in the influent of the treatment plant, E3 was detected occasionally. The concentrations of E1 and E2 ranged from 3 to 50 ng/L, and for E3 from 98 to 210 ng/L. EE2, BPA, and NP could not be detected at the given LOD. In every second surface water sample, El and E2 were detected, but not E3, EE2, BPA, and NP.

Harvard-Zitierstil: Klingelhöfer, I. and Morlock, G. (2015) Bioprofiling of Surface/Wastewater and Bioquantitation of Discovered Endocrine-Active Compounds by Streamlined Direct Bioautography, Analytical Chemistry, 87(21), pp. 11098-11104. https://doi.org/10.1021/acs.analchem.5b03233

APA-Zitierstil: Klingelhöfer, I., & Morlock, G. (2015). Bioprofiling of Surface/Wastewater and Bioquantitation of Discovered Endocrine-Active Compounds by Streamlined Direct Bioautography. Analytical Chemistry. 87(21), 11098-11104. https://doi.org/10.1021/acs.analchem.5b03233