Content of carbohydrates in tropical rainforest nectars of Marantaceae using high-performance thin-layer chromatography - Research portal of Justus Liebig University Giessen:

Journal article

Content of carbohydrates in tropical rainforest nectars of Marantaceae using high-performance thin-layer chromatography

Authors list: Ruppel, A.; Morlock, G.E.

Publication year: 2015

Pages: 162-166

Journal: JPC – Journal of Planar Chromatography – Modern TLC

Volume number: 28

Issue number: 2

DOI Link: https://doi.org/10.1556/JPC.28.2015.2.13

Publisher: Akadémiai Kiadó

Abstract:

The carbohydrate content of floral nectars of 12 Marantaceae species was analyzed by high-performance thin-layer chromatography (HPTLC). The main interest was not only to determine the sugar content of the nectar produced by the flowers but also to find potential differences within the genera and species. A total of 131 samples of this plant family, which grows in the tropical understory rainforest, were collected in the Golfo Dulce Region, Costa Rica, and analyzed. The nectar sugars found in the neotropical samples comprised melezitose, fructose, sucrose, glucose, and xylose (listed ascending in hR(F) values), whereof the main sugars were sucrose, glucose, and fructose. The nectars were successfully analyzed with regard to their individual sugar composition and classified according to their ratios. The overall mean sugar content of the studied species was 36%. The highest sugar contents of >= 40% were found in the nectar of the species Calathea lutea, Calathea donnell-smithii, Calathea marantifolia, Hylaeanthe hoffmannii, and Pleiostachya pruinosa. All species studied showed a ratio of sucrose to glucose and fructose between 1.3 for Calathealutea and 11.2 for Calathea donnell-smithii and, hence, were classified as sucrose dominated. HPTLC was found to be a good analytical method for nectar analysis, which took less than 1.5 h for 21 samples analyzed in parallel (ca. 4 min per sample).

Authors/Editors

- Uni.-Prof. Dr. Gertrud Elisabeth Morlock

Citation Styles

Harvard Citation style: Ruppel, A. and Morlock, G. (2015) Content of carbohydrates in tropical rainforest nectars of Marantaceae using high-performance thin-layer chromatography, JPC – Journal of Planar Chromatography – Modern TLC, 28(2), pp. 162-166. https://doi.org/10.1556/JPC.28.2015.2.13

APA Citation style: Ruppel, A., & Morlock, G. (2015). Content of carbohydrates in tropical rainforest nectars of Marantaceae using high-performance thin-layer chromatography. JPC – Journal of Planar Chromatography – Modern TLC. 28(2), 162-166. https://doi.org/10.1556/JPC.28.2015.2.13

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