Journalartikel

Jahr der Veröffentlichung: 2014

Seiten: 2892-2914

Zeitschrift: Journal of Liquid Chromatography and Related Technologies

Bandnummer: 37

Heftnummer: 20

DOI Link: https://doi.org/10.1080/10739149.2014.907000

Verlag: Taylor and Francis Group

Abstract: 

Among the different thin-layer and high-performance thin-layer chromatography mass spectrometry (TLC/HPTLC-MS) approaches, the elution head-based TLC-MS Interface is increasingly used due to its good detectability, reliability, and versatility in its application. The influence of different solvents, either used for prewashing or chromatography, on the mass signal background was studied. It was shown that the use of acidic solvents can cause intense background signals (acid sodium clusters) and ion suppression in the analyte mass spectra. Using a mixture of methanol-water 3:1 (v/v) for plate prewashing, background mass signals could be reduced to a minimum for both ionization modes. The TLC/HPTLC-MS background mass signals obtained from different plates with the green manganese activated zinc silicate fluorescence indicator F-254, and without, did not show substantial differences. As sodium is ubiquitary in solvent, tubing, glassware, adsorbent, and so forth, the sodium adduct was often formed and observed, whereby the extent of formation was different depending on the plate type. Recommendations for plate handling for use in TLC/HPTLC-MS are provided, as well as practical advices with regard to integration of the inline filter, various sorts of pressure increase, and leakage using the TLC-MS Interface.

Harvard-Zitierstil: Morlock, G. (2014) Background mass signals in tlc/hptlc-esi-ms and practical advices for use of the tlc-ms interface, Journal of Liquid Chromatography and Related Technologies, 37(20), pp. 2892-2914. https://doi.org/10.1080/10739149.2014.907000

APA-Zitierstil: Morlock, G. (2014). Background mass signals in tlc/hptlc-esi-ms and practical advices for use of the tlc-ms interface. Journal of Liquid Chromatography and Related Technologies. 37(20), 2892-2914. https://doi.org/10.1080/10739149.2014.907000