Journal article

Publication year: 2013

Pages: 8657-8665

Journal: Romanian Biotechnological Letters

Volume number: 18

Issue number: 5

URL: https://e-repository.org/rbl/vol.18/iss.5/11.pdf

Publisher: University of Bucharest

Abstract: 

The present study investigates a quantitative high performance thin layer chromatographic (HPTLC) method for chlorogenic acid and hyperoside determination from berry extracts. Chlorogenic acid and hyperoside were extracted using a methanol and water mixture and separated on a HPTLC 60F(254) silica gel plates with ethyl acetate, acetic acid, formic acid and water 10:1.1:1.1:2.3 (v/v/v/v) as mobile phase within 35 minutes. After chromatography, the plates were sprayed with natural products (polyethylene glycol reagent (NP/PEG)). Densitometric analysis of these compounds was performed via fluorescence, under UV light at lambda = 366 nm. The R-f of chlorogenic acid and hyperoside was 0.74 and respectively 0.85. Calibration data revealed good linear and polynomial relationships (r = 0.99996 and 0.99999) between peak height area and chlorogenic acid, respectively hyperoside concentration. Based on the results obtained in this study, the investigated method, can be used for rapid routine analysis of chlorogenic acid and hyperoside.

Harvard Citation style: Cretu, G., Morlock, G., Miron, A. and Nechifor, A. (2013) A high-performance thin-layer chromatographic method for chlorogenic acid and hyperoside determination from berry extracts, Romanian Biotechnological Letters, 18(5), pp. 8657-8665. https://e-repository.org/rbl/vol.18/iss.5/11.pdf

APA Citation style: Cretu, G., Morlock, G., Miron, A., & Nechifor, A. (2013). A high-performance thin-layer chromatographic method for chlorogenic acid and hyperoside determination from berry extracts. Romanian Biotechnological Letters. 18(5), 8657-8665. https://e-repository.org/rbl/vol.18/iss.5/11.pdf