Journal article

Publication year: 2012

Pages: 1364-1387

Journal: Journal of Liquid Chromatography and Related Technologies

Volume number: 35

Issue number: 10

DOI Link: https://doi.org/10.1080/10826076.2012.675859

Publisher: Taylor and Francis Group

Abstract: 

The high-performance thin-layer chromatographic (HPTLC) profiling of sugars, sugar alcohols, amino acids, flavonoids, and phenolic acids, as well as bioactive and antioxidative compounds, is described for the first time in aqueous and hydroalcoholic extracts of some Indian herbs, namely, Hippophae rhamnoides, Valeriana wallichii, Triticum aestivum, and the fungus Cordyceps sinensis, all known for their natural performance enhancement capacity. For the analysis of bioactive compounds such as flavonoids and phenolic acids, the Automated Multiple Development (AMD2) system was employed. For the determination of the bioactivity of the extracts, chromatography was linked to a bioassay, and, therefore, the developed plate was automatically dipped into the luminescent Vibrio fischeri bacteria suspension. The presence of sugars could be confirmed in all aqueous and hydroalcoholic extracts, except for the hydroalcoholic extract of Cordyceps sinensis. Relatively high amounts of amino acids were found in the extracts of Triticum aestivum and Cordyceps sinensis. In terms of sugar alcohols, the highest amount was observed in aqueous and hydroalcoholic extracts of Cordyceps sinensis, in which mannitol was found. Xylitol was present to a high extent in all three extracts of Valeriana wallichii. A high content of flavonoids, phenolic acids, and other bioactive compounds was observed in the hydroalcoholic extract of Valeriana wallichii. Using the DPPH center dot radical reagent, the antioxidant potential of the four extracts was in the following order: Valeriana wallichii hydroalcoholic extract>Triticum aestivum hydroalcoholic extract>Valeriana wallichii aqueous extract>Triticum aestivum aqueous extract>Hippophae rhamnoides aqueous extract>Cordyceps sinensis aqueous extract > Cordyceps sinensis hydroalcoholic extract.

Harvard Citation style: Misra, K., Tulsawani, R., Shyam, R., Meena, D. and Morlock, G. (2012) Hyphenated high-performance thin-layer chromatography for profiling of some Indian natural efficiency enhancers, Journal of Liquid Chromatography and Related Technologies, 35(10), pp. 1364-1387. https://doi.org/10.1080/10826076.2012.675859

APA Citation style: Misra, K., Tulsawani, R., Shyam, R., Meena, D., & Morlock, G. (2012). Hyphenated high-performance thin-layer chromatography for profiling of some Indian natural efficiency enhancers. Journal of Liquid Chromatography and Related Technologies. 35(10), 1364-1387. https://doi.org/10.1080/10826076.2012.675859