Journalartikel

Jahr der Veröffentlichung: 2013

Seiten: 7195-7203

Zeitschrift: Analytical and Bioanalytical Chemistry

Bandnummer: 405

Heftnummer: 23

DOI Link: https://doi.org/10.1007/s00216-013-7131-7

Verlag: Springer

Abstract: 

Ultrathin-layer chromatography (UTLC) potentially offers faster analysis, reduced solvent and sample volumes, and lower costs. One novel technique for producing UTLC plates has been glancing angle deposition (GLAD), a physical vapor deposition technique capable of aligning macropores to produce interesting separation properties. To date, however, GLAD-UTLC plates have been restricted to model dye systems, rather than realistic analytes. This study demonstrates the transfer of high-performance thin-layer chromatography (HPTLC) sugar analysis methods to GLAD-UTLC plates using the office chromatography framework. A consumer inkjet printer was used to apply very sharp low volume (3-30 nL) bands of water-soluble analytes (lactose, sucrose, and fructose). Analytic performance measurements extrapolated the limits of detection to be 3-5 ng/zone, which was experimentally proven down to 60-70 ng/band, depending on the sugar. This qualitative analysis of sugars in a commercially available chocolate sample is the first reported application of GLAD-UTLC to food samples. The potential utility of GLAD-UTLC is further exemplified by successful coupling with electrospray ionization mass spectrometry for the first time to characterize underivatized sugars.

Harvard-Zitierstil: Kirchert, S., Wang, Z., Taschuk, M., Jim, S., Brett, M. and Morlock, G. (2013) Inkjet application, chromatography, and mass spectrometry of sugars on nanostructured thin films, Analytical and Bioanalytical Chemistry, 405(23), pp. 7195-7203. https://doi.org/10.1007/s00216-013-7131-7

APA-Zitierstil: Kirchert, S., Wang, Z., Taschuk, M., Jim, S., Brett, M., & Morlock, G. (2013). Inkjet application, chromatography, and mass spectrometry of sugars on nanostructured thin films. Analytical and Bioanalytical Chemistry. 405(23), 7195-7203. https://doi.org/10.1007/s00216-013-7131-7