Journalartikel

Jahr der Veröffentlichung: 2009

Seiten: 725-729

Zeitschrift: Journal of AOAC INTERNATIONAL

Bandnummer: 92

Heftnummer: 3

URL: https://aoac.publisher.ingentaconnect.com/contentone/aoac/jaoac/2009/00000092/00000003/art00007

Verlag: Oxford University Press

Abstract: 

A new method has been developed for the determination of acrylamide in ground coffee by planar chromatography using prechromatographic in situ derivatization with dansulfinic acid. After pressurized fluid extraction of acrylamide from the coffee samples, the extracts were passed through activated carbon and concentrated. These extracts were applied onto a silica gel 60 HPTLC plate and oversprayed with dansulfinic acid. By heating the plate, acrylamide was derivatized into the fluorescent product dansylpropanamide. The chromatographic separation with ethyl acetatetert.-butyl methyl ether (8 + 2, v/v) mobile phase was followed by densitometric quantification at 254/>400 nm using a 4 point calibration via the standard addition method over the whole system for which acrylamide was added at different concentrations at the beginning of the extraction process. The method was validated for commercial coffee. The linearity over the whole procedure showed determination coefficients between 0.9995 and 0.9825 (n = 6). Limit of quantitation at a signal-to-noise ratio of 10 was determined to be 48 mu g/kg. The within-run precision (relative standard deviation, n = 6) of the chromatographic method was 3%. Commercial coffee samples analyzed showed acrylamide contents between 52 and 191 mu g/kg, which was in correlation with amounts reported in previous publications.

Harvard-Zitierstil: Alpmann, A. and Morlock, G. (2009) Rapid and cost-effective determination of acrylamide in coffee by planar chromatography and fluorescence detection after derivatization with dansulfinic acid, Journal of AOAC INTERNATIONAL, 92(3), pp. 725-729. https://aoac.publisher.ingentaconnect.com/contentone/aoac/jaoac/2009/00000092/00000003/art00007

APA-Zitierstil: Alpmann, A., & Morlock, G. (2009). Rapid and cost-effective determination of acrylamide in coffee by planar chromatography and fluorescence detection after derivatization with dansulfinic acid. Journal of AOAC INTERNATIONAL. 92(3), 725-729. https://aoac.publisher.ingentaconnect.com/contentone/aoac/jaoac/2009/00000092/00000003/art00007