Journalartikel

Jahr der Veröffentlichung: 2007

Seiten: 251-255

Zeitschrift: Journal of Chromatographic Science

Bandnummer: 45

Heftnummer: 5

DOI Link: https://doi.org/10.1093/chromsci/45.5.251

Verlag: Oxford University Press

Abstract: 

A new high-throughput method is developed to quantify caffeine, ergotamine, and metamizol in a solid pharmaceutical formulation. After dissolution, the compounds are separated on silica gel 60 F254 high-performance thin-layer chromatography (HPTLC) plates with ethyl acetate—methanol—ammonia 90:15:1 (v/v/v) as the mobile phase. Detection is performed by UV absorption at 274 nm for caffeine and metamizol, and by fluorescence at 313 /> 340 nm for ergotamine. Calibrations are linear or polynomial with determination coefficients (R2) ≥ 0.9986. Recoveries of the three compounds are between 95% and 102% at three different concentration levels. Repeatability [relative standard deviation (RSD)] of all substances in the matrix is between ± 0.9% and ± 1.7%. Intermediate precision (RSD) of the three compounds range from ± 2.0% to ± 3.1%. Mass confirmation is performed by a single quadrupole mass spectrometry in positive electrospray ionization full scan mode for caffeine and ergotamine and in negative mode for metamizol. The results proved that this method is a simple and reliable alternative for routine analysis.

Harvard-Zitierstil: Aranda, M. and Morlock, G. (2007) Simultaneous determination of caffeine, ergotamine, and metamizol in solid pharmaceutical formulation by HPTLC-UV-FLD with mass confirmation by online HPTLC-ESI-MS, Journal of Chromatographic Science, 45(5), pp. 251-255. https://doi.org/10.1093/chromsci/45.5.251

APA-Zitierstil: Aranda, M., & Morlock, G. (2007). Simultaneous determination of caffeine, ergotamine, and metamizol in solid pharmaceutical formulation by HPTLC-UV-FLD with mass confirmation by online HPTLC-ESI-MS. Journal of Chromatographic Science. 45(5), 251-255. https://doi.org/10.1093/chromsci/45.5.251