For determination of the sweetener sucralose in the UV-visible range, derivatization is a necessity because of its weak UV absorption (<= 200 nm). A new HPTLC method on silica gel plates, in which 46 chromatographic runs were performed within 13 min, has been established. Densitometry was performed either at 500 nm after post-chromatographic derivatization with 2-naphthol sulfuric acid reagent (brownish band for sucralose) or at 405 nm after post-chromatographic derivatization with aniline diphenylamine orthophosphoric acid reagent (gray-bluish band for sucralose). With the latter reagent bluish fluorescence at 366/>400 nm was also observed for sucralose; this enabled very sensitive and selective detection comparable with that on amino plates. The new methods based on silica phases were compared with the reagent-free method on amino plates with regard to limit of detection (LOD) of sucralose. LODs on silica gel measured at 366/>400 run, 405 nm, and 500 nm and were 4, 8, and 5 ng per band, respectively. Selectivity for different matrices, for example milk-based cakes, different sorts of biscuit, chocolate, cola, milk, hard candies, and energy or sport drinks was achieved for all the detection modes used. Specificity was even obtained by use of the aniline diphenylamine orthophosphoric acid reagent because only sucralose produced a fluorescent color despite the presence of carbohydrates and other matrix constituents. Finally, the four different modes of detection were compared for analysis of sucralose in dietetic biscuits and cakes. The sucralose content measured was shown to be highly repeatable (RSD <= 4.4%, n = 3) and reliable with regard to the target value of the sucralose labeled on the packages. According to the target value t-test the means obtained were not significantly different from the labeled values of 45.3 mg/100 g for cakes and 24.8 mg/100 g for biscuits. The good accuracy in matrix proves HPTLC is a highly suitable method for quantification of sucralose in dietetic cakes and biscuits, and in a variety of other matrices.