Journalartikel

Jahr der Veröffentlichung: 2019

Seiten: 131-139

Zeitschrift: Analytica Chimica Acta

Bandnummer: 1087

ISSN: 0003-2670

DOI Link: https://doi.org/10.1016/j.aca.2019.08.013

Verlag: Elsevier

Abstract: 
High-performance thin-layer chromatography (HPTLC) combined with H-1 nuclear magnetic resonance (NMR) spectroscopy has only been demonstrated over a small spectral range so far. The self-printing of chromatographic plates with a modified 3D slurry printer allowed the operator to influence the composition of the adsorbent slurry and thus the purity of the adsorbent layer. The combination of such self-printed, binder-free HPTLC plates with inkjet-driven sample application made possible submicromole-scaled analytical H-1 NMR spectroscopy. This was proven using pure HPTLC adsorbents. For comparison, commercial silica gel HPTLC plates were purified by pre-development with solvents for spectroscopy, whereas commercial silica gel HPTLC particles were self-printed on the glass plate after purification under solvent pressure in a recycled HPLC cartridge. Evaluating the signals from different treatments, seven background signals disappeared in the proton spectra and three were reduced to a minimum by use of pre-developed commercial HPTLC plates. In the case of the self-printed, binder-free HPTLC plates made of purified adsorbent, most of the spectral background signals were reduced to a minimum, thus these spectra showed the highest cleanness and most pure analyte proton spectra. For the first time, the full H-1 NMR spectroscopy range was made available after an HPTLC separation. This proof of principle opens the avenue for submicromole-scaled analytical H-1 NMR spectroscopy. (C) 2019 Elsevier B.V. All rights reserved.

Harvard-Zitierstil: Yüce, I., Mayr, M. and Morlock, G. (2019) Quantitative inkjet application on self-printed, binder-free HPTLC layers for submicromole-scaled analytical H-1 NMR spectroscopy, Analytica Chimica Acta, 1087, pp. 131-139. https://doi.org/10.1016/j.aca.2019.08.013

APA-Zitierstil: Yüce, I., Mayr, M., & Morlock, G. (2019). Quantitative inkjet application on self-printed, binder-free HPTLC layers for submicromole-scaled analytical H-1 NMR spectroscopy. Analytica Chimica Acta. 1087, 131-139. https://doi.org/10.1016/j.aca.2019.08.013