Journal article

Publication year: 1997

Pages: 94-103

Journal: Journal of applied botany = Angewandte Botanik

Volume number: 71

Issue number: 3-4

ISSN: 0949-5460

Publisher: Blackwell

Abstract: 
A supplement of an auxin (indol-3-acetic acid or 2,4 dichlorophenoxyacetic acid) to the nutrient medium initiated a differentiation program including rhizogenesis followed by somatic embryogenesis in cultured carrot petiole explants during a cultural cycle of 30 days. The evaluation of the occurrence and the synthesis of 282 soluble proteins extracted from cultured petiole explants during the induction of embryogenesis during the first two weeks of the cultural cycle indicated the switching on and off of protein synthesis sub-programs with a characteristic protein pattern.The concentration of abscisic acid continually decreased during a culture period of 20 days from a high level in the initial explants. The concentration of indol-3-acetic acid reached the highest level at day 6 of the cultural cycle concurrently with the appearance of root primordia, then a decrease followed. Using transgenic plant material (MAS-promoter coupled to GUS-reporter gene) the distribution of IAA was followed by histochemistry. Total cytokinin concentration increased from a low level in the original petiole explants up to day 10, which coincides with the development of embryogenic cells in the sub-epidermis which was followed by lower concentrations during further embryo development. Of the 6 cytokinins determined (2-isopentenyladenine, zeatin and dihydrozeatin and their ribosides), 2-isopentenyladenine and its riboside clearly dominate at all stages of the cultural cycle analyzed.

Harvard Citation style: Grieb, B., Schäfer, F., Imani, J., Mashayekhi, K., Arnholdt-Schmitt, B. and Neumann, K. (1997) Changes in soluble proteins and phytohormone concentrations of cultured carrot petiole explants during induction of somatic embryogenesis (Daucus carota L.), Journal of applied botany = Angewandte Botanik, 71(3-4), pp. 94-103

APA Citation style: Grieb, B., Schäfer, F., Imani, J., Mashayekhi, K., Arnholdt-Schmitt, B., & Neumann, K. (1997). Changes in soluble proteins and phytohormone concentrations of cultured carrot petiole explants during induction of somatic embryogenesis (Daucus carota L.). Journal of applied botany = Angewandte Botanik. 71(3-4), 94-103.