Journal article

Somatic embryogenesis and DNA organization of genomes from selected Daucus species


Authors listImani, J; Thi, LT; Langen, G; Arnholdt-Schmitt, B; Roy, S; Lein, C; Kumar, A; Neumann, KH

Publication year2001

Pages537-541

JournalPlant Cell Reports

Volume number20

Issue number6

ISSN0721-7714

PublisherSpringer


Abstract
Under identical culture conditions, only 8 out of 12 species and subspecies of the genus Daucus proved capable of somatic embryogenesis. Random amplified polymorphic DNA analysis indicated a polymorphism between the genomes of individual species that were capable of embryogenesis and those that were not. Two specific bands (1.1 kbp, 0.68 kbp) were detected only in the genomes of individuals with the capacity for embryogenesis. These were cloned and sequenced, and the homology of the nucleotide sequences of the various species was detected: this ranged from 74% to 92% for the larger sequence and from 92% to 97% for the smaller one. These DNA sequences would appear to be useful as a marker of the capacity for somatic embryogenesis in the genus Daucus.



Citation Styles

Harvard Citation styleImani, J., Thi, L., Langen, G., Arnholdt-Schmitt, B., Roy, S., Lein, C., et al. (2001) Somatic embryogenesis and DNA organization of genomes from selected Daucus species, Plant Cell Reports, 20(6), pp. 537-541

APA Citation styleImani, J., Thi, L., Langen, G., Arnholdt-Schmitt, B., Roy, S., Lein, C., Kumar, A., & Neumann, K. (2001). Somatic embryogenesis and DNA organization of genomes from selected Daucus species. Plant Cell Reports. 20(6), 537-541.



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