Journal article

Publication year: 2001

Pages: 188-194

Journal: Journal of applied botany = Angewandte Botanik

Volume number: 75

Issue number: 5-6

ISSN: 0949-5460

Publisher: Blackwell

Abstract: 
The induction of somatic embryogenesis in petiole explants of Daucus carota (cv. Rotin) requires besides an auxin also a supplement of carbohydrates (GRIEB et al., 1994). During the induction phase of 14 days a maximum of IAA concentration was observed after six days of culture (GRIEB et al., 1997). At this stage rhizogenic centers occur near vascular bundles (LI and NEUMANN, 1985) and here an accumulation of IAA was detected histochemically (GRIEB et al., 1997; IMANt, 1999). Concomitantly starch begins to accumulate. Starch is deposited in cells adjacent to vascular bundles, however, at the end of the induction phase accumulated starch can disappear almost completely.As it was shown recently (GRIEB et al., 1997) de-novo protein synthesis occurs predominantly in active morphogenic areas of cultured petiole explants. During the second week of the induction phase the synthesis of proteins analog to enzymes related to carbohydrate metabolism can be detected.It is shown that besides the hormone system exogenous carbohydrates arc probably involved in the regulation of somatic embryogenesis. From the results reported it is concluded that the hexokinase action is essential for somatic embryogensis but not the products of it's activity.

Harvard Citation style: Pleschka, E., Mashayekhi-Nezamabadi, K., Imani, J., Lein, C. and Neumann, K. (2001) The significance of carbohydrates in somatic embryogenesis of carrot tissue cultures, Journal of applied botany = Angewandte Botanik, 75(5-6), pp. 188-194

APA Citation style: Pleschka, E., Mashayekhi-Nezamabadi, K., Imani, J., Lein, C., & Neumann, K. (2001). The significance of carbohydrates in somatic embryogenesis of carrot tissue cultures. Journal of applied botany = Angewandte Botanik. 75(5-6), 188-194.