Journal article

Publication year: 2009

Pages: 4105-4114

Journal: Journal of Experimental Botany

Volume number: 60

Issue number: 14

ISSN: 0022-0957

Open access status: Hybrid

DOI Link: https://doi.org/10.1093/jxb/erp240

Publisher: Oxford University Press

Abstract: 
The potential of metchnikowin, a 26-amino acid residue proline-rich antimicrobial peptide synthesized in the fat body of Drosophila melanogaster was explored to engineer disease resistance in barley against devastating fungal plant pathogens. The synthetic peptide caused strong in vitro growth inhibition (IC50 value similar to 1 mu M) of the pathogenic fungus Fusarium graminearum. Transgenic barley expressing the metchnikowin gene in its 52-amino acid pre-propeptide form under the control of the inducible mannopine synthase (mas) gene promoter from the T-i plasmid of Agrobacterium tumefaciens displayed enhanced resistance to powdery mildew as well as Fusarium head blight and root rot. In response to these pathogens, metchnikowin accumulated in plant apoplastic space, specifying that the insect signal peptide is functional in monocotyledons. In vitro and in vivo tests revealed that the peptide is markedly effective against fungal pathogens of the phylum Ascomycota but, clearly, less active against Basidiomycota fungi. Importantly, germination of the mutualistic basidiomycete mycorrhizal fungus Piriformospora indica was affected only at concentrations beyond 50 mu M. These results suggest that antifungal peptides from insects are a valuable source for crop plant improvements and their differential activities toward different phyla of fungi denote a capacity for insect peptides to be used as selective measures on specific plant diseases.

Harvard Citation style: Rahnamaeian, M., Langen, G., Imani, J., Khalifa, W., Altincicek, B., von Wettstein, D., et al. (2009) Insect peptide metchnikowin confers on barley a selective capacity for resistance to fungal ascomycetes pathogens, Journal of Experimental Botany, 60(14), pp. 4105-4114. https://doi.org/10.1093/jxb/erp240

APA Citation style: Rahnamaeian, M., Langen, G., Imani, J., Khalifa, W., Altincicek, B., von Wettstein, D., Kogel, K., & Vilcinskas, A. (2009). Insect peptide metchnikowin confers on barley a selective capacity for resistance to fungal ascomycetes pathogens. Journal of Experimental Botany. 60(14), 4105-4114. https://doi.org/10.1093/jxb/erp240