Journal article

Publication year: 2009

Pages: 455-463

Journal: Theoretical and Applied Genetics

Volume number: 118

Issue number: 3

ISSN: 0040-5752

DOI Link: https://doi.org/10.1007/s00122-008-0912-2

Publisher: Springer

Abstract: 
BAX inhibitor-1 (BI-1) is a conserved cell death regulator protein that inhibits mammalian BAX-induced cell death in yeast, animals and plants. Additionally, HvBI-1 suppresses defense responses and resistance to the powdery mildew fungus Blumeria graminis f.sp. hordei (Bgh) when over-expressed in single epidermal cells of barley. To test the potential of ectopic expression of BI-1 to influence fungal interactions with crop plants, we produced stable transgenic barley plants expressing a green fluorescing protein (GFP) fusion of HvBI-1 under control of the cauliflower mosaic virus 35S promoter. GFP-HvBI-1 plants were fertile and did not display obvious developmental alterations when compared to wild type parents. GFP-HvBI-1 plants were more resistant to single cell death induced by ballistic delivery of a mammalian proapototic BAX expression construct and more susceptible to biotrophic Bgh. Microscopic observation of the interaction phenotype revealed that enhanced susceptibility, i.e. a higher degree of successful establishment of haustoria in epidermal cells, was associated with a reduced frequency of hypersensitive cell death reactions. In contrast, young seedlings of GFP-HvBI-1 barley were more resistant to Fusarium graminearum than wild type or azygous controls. Hence the effect of GFP-HvBI-1 on the outcome of a particular plant-fungus interaction appeared dependent on the lifestyle of the pathogen.

Harvard Citation style: Babaeizad, V., Imani, J., Kogel, K., Eichmann, R. and Hückelhoven, R. (2009) Over-expression of the cell death regulator BAX inhibitor-1 in barley confers reduced or enhanced susceptibility to distinct fungal pathogens, TAG Theoretical and Applied Genetics, 118(3), pp. 455-463. https://doi.org/10.1007/s00122-008-0912-2

APA Citation style: Babaeizad, V., Imani, J., Kogel, K., Eichmann, R., & Hückelhoven, R. (2009). Over-expression of the cell death regulator BAX inhibitor-1 in barley confers reduced or enhanced susceptibility to distinct fungal pathogens. TAG Theoretical and Applied Genetics. 118(3), 455-463. https://doi.org/10.1007/s00122-008-0912-2