Journalartikel

Jahr der Veröffentlichung: 2016

Seiten: 2461-2468

Zeitschrift: Endocrinology

Bandnummer: 157

Heftnummer: 6

ISSN: 0013-7227

DOI Link: https://doi.org/10.1210/en.2015-1966

Verlag: Oxford University Press

Abstract: 
Obesity has recently been linked with reduced fertility, and the mechanisms underpinning this effect are currently unknown. The adipokine leptin is dysregulated in obesity and affects reproductive tracts; therefore, we investigated the dose-dependent effects of leptin on Leydig cell function and spermatogenesis. Eight-week-old leptin-deficient obese (ob/ob) male mice were treated with subphysiological (0.1-or 0.5-mg/kg body weight [BW]/d) or physiological (3.0-mg/kg BW/d) doses of leptin or saline for 12 weeks (chronic treatment) or 72 hours (acute treatment). We then evaluated male reproductive function markers. Mean testis weight increased significantly in the 0.1- and 3.0-mg/kg BW/d groups compared with saline controls (both P<.05). Intratesticular testosterone levels relative to testis weight significantly increased in the 0.5-mg/kg BW/d group compared with saline controls (P<.05). FSH levels increased in a dose-dependent manner with leptin treatment, whereas LH levels did not change. Leptin treatment significantly up-regulated both mRNA and protein expression of the steroidogenic enzyme cytochrome P450 17A1. Spermatogenesis improved in leptin-treated animals. Significantly more seminiferous tubules were observed in stages I-VIII (P<.01), and there were fewer abnormal seminiferous tubule structures (P<.01). Acute treatment with physiological leptin doses partially improved male reproductive markers without changing BW. Administration of subphysiological to physiological doses of leptin improves Leydig cell function and spermatogenesis.

Harvard-Zitierstil: Hoffmann, A., Manjowk, G., Wagner, I., Klöting, N., Ebert, T., Jessnitzer, B., et al. (2016) Leptin Within the Subphysiological to Physiological Range Dose Dependently Improves Male Reproductive Function in an Obesity Mouse Model, Endocrinology, 157(6), pp. 2461-2468. https://doi.org/10.1210/en.2015-1966

APA-Zitierstil: Hoffmann, A., Manjowk, G., Wagner, I., Klöting, N., Ebert, T., Jessnitzer, B., Lössner, U., Stukenborg, J., Blüher, M., Stumvoll, M., Söder, O., Svechnikov, K., Fasshauer, M., & Kralisch, S. (2016). Leptin Within the Subphysiological to Physiological Range Dose Dependently Improves Male Reproductive Function in an Obesity Mouse Model. Endocrinology. 157(6), 2461-2468. https://doi.org/10.1210/en.2015-1966