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Journal article

Circulating Adipocyte Fatty Acid-Binding Protein Induces Insulin Resistance in Mice In Vivo

Authors list: Kralisch, S; Klöting, N; Ebert, T; Kern, M; Hoffmann, A; Krause, K; Jessnitzer, B; Lössner, U; Sommerer, I; Stumvoll, M; Fasshauer, M

Publication year: 2015

Pages: 1007-1013

Journal: Obesity

Volume number: 23

Issue number: 5

ISSN: 1930-7381

DOI Link: https://doi.org/10.1002/oby.21057

Publisher: Wiley

Abstract:
ObjectiveCirculating levels of the adipokine adipocyte fatty acid-binding protein (AFABP) are increased in obesity. However, the influence of circulating AFABP on insulin sensitivity in vivo remains unclear.MethodsC57BL/6NTac mice (10 weeks) were treated over 8 weeks i.p. with saline (control) or recombinant AFABP (0.5 mg/kg/d). A comprehensive characterization of metabolic parameters, body composition, and energy expenditure was performed. Furthermore, the effect of AFABP on pancreatic -cell responsiveness, hepatic glycogen content, and peroxisome proliferator-activated receptor (PPAR) protein expression was elucidated.ResultsIn male mice, AFABP treatment induced insulin resistance with significantly increased fasting insulin, C-peptide, and homeostasis model assessment of insulin resistance. In female animals, a similar trend was observed. In both genders, no difference in body weight, lipid parameters, body composition, or energy expenditure could be detected between AFABP-treated and control mice. Insulin resistance in male AFABP-treated mice was accompanied by decreased PPAR protein content in perigonadal adipose tissue and diminished circulating adiponectin. AFABP treatment did not affect pancreatic -cell responsiveness and hepatic glycogen content.ConclusionsCirculating AFABP induces insulin resistance in male mice. AFABP-mediated degradation of PPAR in adipose tissue and subsequently decreased expression of insulin-sensitizing adiponectin are potential mechanisms for this effect.

Authors/Editors

- Uni.-Prof. Dr. Mathias Faßhauer

Citation Styles

Harvard Citation style: Kralisch, S., Klöting, N., Ebert, T., Kern, M., Hoffmann, A., Krause, K., et al. (2015) Circulating Adipocyte Fatty Acid-Binding Protein Induces Insulin Resistance in Mice In Vivo, Obesity, 23(5), pp. 1007-1013. https://doi.org/10.1002/oby.21057

APA Citation style: Kralisch, S., Klöting, N., Ebert, T., Kern, M., Hoffmann, A., Krause, K., Jessnitzer, B., Lössner, U., Sommerer, I., Stumvoll, M., & Fasshauer, M. (2015). Circulating Adipocyte Fatty Acid-Binding Protein Induces Insulin Resistance in Mice In Vivo. Obesity. 23(5), 1007-1013. https://doi.org/10.1002/oby.21057