Journal article

Publication year: 2009

Pages: 1418-1422

Journal: Journal of Cellular Biochemistry

Volume number: 108

Issue number: 6

ISSN: 0730-2312

DOI Link: https://doi.org/10.1002/jcb.22382

Publisher: Wiley

Abstract: 
Amyloid precursor protein (APP) has been characterized as an adipocyte-secreted protein that might contribute to obesity-related insulin resistance, inflammation, and dementia. In the current study, regulation of APP by the proinflammatory and insulin resistance-inducing cytokine tumor necrosis factor (TNF) alpha was determined in 3T3-L1 adipocytes. Interestingly, APP protein synthesis and mRNA expression were significantly increased by TNF alpha in a time-dependent manner with maximal induction observed after 24 h of treatment. Furthermore, TNF alpha induced APP mRNA expression dose-dependently with maximal 6.4-fold upregulation seen at 100 ng/ml effector. Moreover, inhibitor experiments suggested that TNF alpha-induced APP expression was mediated by nuclear factor kappa B. Taken together, we show for the first time a potent upregulation of APP by TNF alpha suggesting a potential role of this adipocyte-secreted protein in TNF alpha-induced insulin resistance and inflammatory disease. J. Cell. Biochem. 108: 1418-1422, 2009. (C) 2009 Wiley-Liss, Inc.

Harvard Citation style: Sommer, G., Kralisch, S., Lipfert, J., Weise, S., Krause, K., Jessnitzer, B., et al. (2009) Amyloid Precursor Protein Expression Is induced by Tumor Necrosis Factor alpha in 3T3-L1 Adipocytes, Journal of Cellular Biochemistry, 108(6), pp. 1418-1422. https://doi.org/10.1002/jcb.22382

APA Citation style: Sommer, G., Kralisch, S., Lipfert, J., Weise, S., Krause, K., Jessnitzer, B., Lössner, U., Blüher, M., Stumvoll, M., & Fasshauer, M. (2009). Amyloid Precursor Protein Expression Is induced by Tumor Necrosis Factor alpha in 3T3-L1 Adipocytes. Journal of Cellular Biochemistry. 108(6), 1418-1422. https://doi.org/10.1002/jcb.22382